Reis D J, Joh T H, Ross R A
J Pharmacol Exp Ther. 1975 Jun;193(3):775-84.
Reserpine (10 mg/kp i.p.) increased the activities of the enzymes tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase, but not dopa decarboxylase, 2- to 3-fold in the nucleus locus ceruleus of rat brain. The TH response was dose-dependent, reached a maximum by 48 hours and recovered by 3 weeks. The increased activities of TH and dopamine-beta-hydroylase were shown by immunotitration with specific antibodies to each enzyme to be due entirely to increased accumulation of specific enzyme protein. At a time when the reserpine-elicited accumulation of TH was increased 300% in the locus ceruleus, activity of the enzyme only increased 20% in hypothalamus and not at all in substantia nigra or caudate nucleus. Reserpine increased the accumulation of TH, primarily in the cell bodies and to a far less degree in the terminals of neurons of locus ceruleus but not in dopaminergic neurons of the nigrostriatal system. The time course of enzyme accumulation parallels that of depletion of monoamines in brain.
利血平（10毫克/千克，腹腔注射）使大鼠脑海马蓝斑核中酪氨酸羟化酶（TH）和多巴胺-β-羟化酶的活性增加了2至3倍，但对多巴脱羧酶活性没有影响。TH反应呈剂量依赖性，48小时达到最大值，3周后恢复。通过用针对每种酶的特异性抗体进行免疫滴定表明，TH和多巴胺-β-羟化酶活性的增加完全是由于特异性酶蛋白积累的增加。当利血平引起的海马蓝斑核中TH积累增加300%时，该酶在下丘脑的活性仅增加20%，在黑质或尾状核中则完全没有增加。利血平增加了TH的积累，主要在海马蓝斑核神经元的细胞体中，在其终末的增加程度要小得多，而在黑质纹状体系统的多巴胺能神经元中则没有增加。酶积累的时间进程与脑中单胺耗竭的时间进程相似。
