Letournel-Boulland M L, Fages C, Rolland B, Tardy M
INSERM U-282, Hôpital Henri Mondor, Creteil, France.
Eur Cytokine Netw. 1994 Jan-Feb;5(1):51-6.
The effect of lipopolysaccharides (LPS), a component of gram-negative bacteria, has been studied in both exponentially growing and confluent morphologically differentiated astroglial cells in primary cultures. The expression of glial fibrillary acidic protein (GFAP) and Glutamine Synthetase (GS) were investigated in parallel with proliferation and expression of IL-1 beta-mRNA. During the exponential growth, proliferation was severely inhibited by LPS. The effect was time- and dose-dependent. On confluent differentiated cells LPS induced an inhibition of cell proliferation which was associated with a down-regulation of GFAP-mRNA, GS-mRNA and GS expressions and with a transitory increase in IL-1 beta mRNA expression. The observed effects might interact with the astroglial developmental program and with the astroglial function.
革兰氏阴性菌的一种成分脂多糖（LPS）在原代培养中指数生长和汇合的形态分化星形胶质细胞中的作用已得到研究。同时研究了胶质纤维酸性蛋白（GFAP）和谷氨酰胺合成酶（GS）的表达与白细胞介素1β（IL-1β）-mRNA的增殖和表达情况。在指数生长期间，LPS严重抑制增殖。该作用具有时间和剂量依赖性。在汇合的分化细胞上，LPS诱导细胞增殖受到抑制，这与GFAP-mRNA、GS-mRNA和GS表达的下调以及IL-1β mRNA表达的短暂增加有关。观察到的这些作用可能与星形胶质细胞的发育程序以及星形胶质细胞功能相互作用。
