Khlusov I A, Dygaĭ A M, Aksinenko S G, Gol'dberg E D
Biull Eksp Biol Med. 1993 Apr;115(4):372-5.
The role of the sympathetic nervous system in the regulation of bone marrow hematopoiesis has been studied on mice CBA after administration of 5-fluorouracil (114 mg/kg). The double injection (3-5 minutes before and 5 hours after 5 + U) of ganglionic blocking drug (pentamine, 6 mg/kg), alpha-adrenergic (dihydroergotamine, 3.9 mg/kg) or beta-adrenergic antagonists (propranolol, 5 mg/kg) inhibited the post-cytostatic reparation of erythro- and granulocytopoiesis. Besides, addition of the same neurotropic antagonists and 5-FU (10(-7) M) to bone marrow culture led to 1.5-3 fold decrease of cell content in comparison with alone cytostatic after 24 hours incubation. On the contrary injection of neuroblocking agents (twice in 5 hours) on day 3 after 5-FU administration elevated the total count of erythroid cells immature and mature granulocytic leukocytes in bone marrow and polymorphonuclear leukocytes in blood. Thus, the unequal role of the sympathetic nervous system in the regulation of hematopoiesis at the different periods after high dose of 5-FU administration has been demonstrated.
在给CBA小鼠注射5-氟尿嘧啶（114毫克/千克）后，研究了交感神经系统在调节骨髓造血中的作用。在注射5-氟尿嘧啶前后分别注射神经节阻断药物（喷他明，6毫克/千克）、α-肾上腺素能拮抗剂（双氢麦角胺，3.9毫克/千克）或β-肾上腺素能拮抗剂（普萘洛尔，5毫克/千克）（分别在注射5-氟尿嘧啶前3-5分钟和后5小时），可抑制细胞生长抑制剂作用后红细胞生成和粒细胞生成的恢复。此外，在骨髓培养物中加入相同的神经亲和拮抗剂和5-氟尿嘧啶（10^(-7) M），与单独使用细胞生长抑制剂相比，孵育24小时后细胞含量降低了1.5至3倍。相反，在注射5-氟尿嘧啶后第3天注射神经阻断剂（5小时内两次），可提高骨髓中未成熟和成熟粒细胞系白细胞以及血液中多形核白细胞的总数。因此，已证明交感神经系统在高剂量5-氟尿嘧啶给药后不同时期对造血调节的作用不同。
