Sindou P, Lesort M, Couratier P, Yardin C, Esclaire F, Hugon J
Unité de Neurobiologie Cellulaire, Faculté de Médecine, Limoges, France.
Brain Res. 1994 May 16;646(1):124-8. doi: 10.1016/0006-8993(94)90064-7.
Tau proteins are microtubule-associated proteins which promote microtubule polymerisation and stabilization. AT8 is a new monoclonal antibody raised against a phosphorylated Tau protein probably at Serine 202. Tau protein, recognized by AT8 antibody is present in fetal human and rat brains, and in Alzheimer's brains. Here we report that glutamate an excitatory neurotransmitter and also a potent excitotoxin produces in primary neuronal cultures a rapid increase in phosphorylated Tau protein immunoreactivity using AT8 antibody. Glutamate augments neuronal Tau immunoreactivity by 225% using laser confocal immunocytochemistry and by 355% on immunoblot analysis. This experimental model of Tau protein modifications could help to decipher the intracellular biochemical pathways at the origin of phosphorylated Tau protein.
tau蛋白是与微管相关的蛋白质，可促进微管聚合和稳定。AT8是一种新的单克隆抗体，针对可能位于丝氨酸202处的磷酸化tau蛋白产生。被AT8抗体识别的tau蛋白存在于人类胎儿和大鼠大脑以及阿尔茨海默病患者的大脑中。在此我们报告，谷氨酸作为一种兴奋性神经递质以及一种强效兴奋性毒素，在原代神经元培养物中使用AT8抗体可使磷酸化tau蛋白免疫反应性迅速增加。通过激光共聚焦免疫细胞化学，谷氨酸可使神经元tau免疫反应性增强225%，免疫印迹分析显示增强355%。这种tau蛋白修饰的实验模型可能有助于解读磷酸化tau蛋白产生的细胞内生化途径。
