Marchi A, Pili E
Istituto di Biologia Generale, Facoltà di Medicina e Chirurgia, Università di Cagliari, Italy.
Heredity (Edinb). 1994 Jun;72 ( Pt 6):599-605. doi: 10.1038/hdy.1994.83.
Fluorescence in situ hybridization (FISH) was used to localize the 18S-28S ribosomal RNA gene clusters on the chromosomes of 15 mosquito species belonging to the Anophelinae and Culicinae subfamilies. In the genus Anopheles the rRNA genes are localized on the heterochromatic arm of both sex chromosomes. The association between rRNA genes and sex determining chromosomes also applies to the homomorphic karyotype of Culicinae mosquitoes, at least in those cases in which localization of the sex locus/loci has been determined. In these species ribosomal genes are often found within or adjacent to heterochromatic regions (C bands). Differences in the location of rRNA genes among and within genera suggest the occurrence of several chromosomal rearrangements during the evolution of mosquitoes.
荧光原位杂交（FISH）被用于定位属于按蚊亚科和库蚊亚科的15种蚊子染色体上的18S - 28S核糖体RNA基因簇。在按蚊属中，rRNA基因定位在两条性染色体的异染色质臂上。rRNA基因与性别决定染色体之间的关联也适用于库蚊亚科蚊子的同形核型，至少在那些已确定性别位点定位的情况下是如此。在这些物种中，核糖体基因常常位于异染色质区域（C带）内或其附近。属间和属内rRNA基因位置的差异表明在蚊子进化过程中发生了几次染色体重排。
