Hide G, Welburn S C, Tait A, Maudlin I
Department of Veterinary Parasitology, Glasgow University Veterinary School.
Parasitology. 1994 Jul;109 ( Pt 1):95-111. doi: 10.1017/s0031182000077805.
This study represents an analysis of trypanosome strains circulating within a confined location over a short period of time during a sleeping sickness epidemic in S.E. Uganda. A large number of Trypanosoma brucei isolates (88) were collected from a variety of hosts (man, cattle, pigs and tsetse) from villages within a 10 km radius and were analysed for variation in isoenzyme patterns, restriction fragment length polymorphism (RFLP) in repetitive DNA sequences and susceptibility to human serum. The human infective stocks form a clearly distinguishable population when compared with other stocks circulating in the domestic cattle reservoir. The data here support the occurrence of genetic exchange between the cattle stocks while an 'epidemic' population structure involving limited genetic exchange is a characteristic of the human infective stocks. Furthermore, it is shown that when both RFLP and isoenzyme analysis are carried out most stocks appear to have individual genotypes. Stocks which were formerly grouped as zymodemes are better considered as a collected of distinct individuals.
本研究对乌干达东南部昏睡病流行期间，在短时间内局限区域内传播的锥虫菌株进行了分析。从半径10公里范围内村庄的各种宿主（人、牛、猪和采采蝇）中收集了大量布氏锥虫分离株（88株），并对其同工酶模式的变异、重复DNA序列中的限制性片段长度多态性（RFLP）以及对人血清的敏感性进行了分析。与在家畜宿主中传播的其他菌株相比，人类感染菌株形成了一个明显可区分的群体。此处的数据支持家畜菌株之间发生了基因交换，而涉及有限基因交换的“流行”群体结构是人类感染菌株的一个特征。此外，研究表明，当同时进行RFLP和同工酶分析时，大多数菌株似乎都有各自的基因型。以前归为酶谱型的菌株，最好看作是不同个体的集合。
