Ricevuti G, Mazzone A, Mazzucchelli I, Fossati G, Pasotti D, Cavigliano P, Rolandi L, Viarengo G, Rossi M, Notario A
Department of Internal Medicine, University of Pavia, IRCCS S. Matteo Hospital, Italy.
FEMS Microbiol Immunol. 1992 Dec;5(5-6):271-8. doi: 10.1111/j.1574-6968.1992.tb05911.x.
Recent studies suggest that granulocytes (PMNs) play a role in the pathogenesis of acute and chronic myocardial ischemia and extension of myocardial injury. Granulocytes can release a variety of molecules mediating tissue injury which act synergistically with other molecules and cells. The aim of our investigation was to evaluate the granulocyte function in patients affected by coronary artery disease (CAD) and during coronary angioplasty (PTCA). We studied 20 patients suffering from CAD. The PMN's aggregating activity was greater in the coronary sinus than in the aorta (P < 0.01). The increase in aggregating activity was evident in patients who were smokers: their cells release significantly lower quantities of leukotriene C4 (P < 0.025). In the 20 patients who underwent coronary angioplasty we analyzed superoxide release after stimulation with phorbol-myristate-acetate (PMA). The results showed a greater decrease of PMN's superoxide production in the coronary sinus than in the aorta (P < 0.05). In all patients affected by CAD we evaluated the PMN's expression of CD11b/CD18 membrane integrins. In these patients the increase in expression of CD11b/CD18 was statistically significant in comparison with the controls (P < 0.01). This increase in expression correlates with a higher aggregation (r = 0.87, P < 0.001). The potential role of leukocytes, oxygen radicals, leukotrienes and granulocyte enzymes in the pathophysiology of myocardial injury due to regional ischemia and reperfusion is an area of intense investigation. This paper presents studies carried out in vivo which have been instrumental in demonstrating the role of granulocytes as mediators of myocardial ischemia.
近期研究表明，粒细胞（多形核白细胞）在急性和慢性心肌缺血的发病机制以及心肌损伤的扩展中发挥作用。粒细胞可释放多种介导组织损伤的分子，这些分子与其他分子和细胞协同作用。我们研究的目的是评估冠心病（CAD）患者以及冠状动脉血管成形术（PTCA）期间的粒细胞功能。我们研究了20例CAD患者。冠状动脉窦中多形核白细胞的聚集活性高于主动脉（P < 0.01）。吸烟者的聚集活性增加明显：他们的细胞释放的白三烯C4量显著更低（P < 0.025）。在接受冠状动脉血管成形术的20例患者中，我们分析了用佛波酯 - 肉豆蔻酸酯 - 乙酸酯（PMA）刺激后的超氧化物释放。结果显示，冠状动脉窦中多形核白细胞的超氧化物产生量比主动脉中的下降幅度更大（P < 0.05）。在所有CAD患者中，我们评估了多形核白细胞CD11b/CD18膜整合素的表达。与对照组相比，这些患者中CD11b/CD18的表达增加具有统计学意义（P < 0.01）。这种表达增加与更高的聚集相关（r = 0.87，P < 0.001）。白细胞、氧自由基、白三烯和粒细胞酶在局部缺血和再灌注导致的心肌损伤病理生理学中的潜在作用是一个深入研究的领域。本文介绍了在体内进行的研究，这些研究有助于证明粒细胞作为心肌缺血介质的作用。
