Knutsen A P, Mueller K R, Levine A D, Chouhan B, Hutcheson P S, Slavin R G
Pediatric Research Institute, St. Louis University Medical Center, MO 63104.
J Allergy Clin Immunol. 1994 Aug;94(2 Pt 1):215-21. doi: 10.1016/0091-6749(94)90043-4.
Allergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity lung disease caused by bronchial colonization with Aspergillus fumigatus (Af) characterized by elevated serum total and Af-specific IgE levels and eosinophilia. In order to examine T-cell reactivity to Af antigens, six T-cell lines were established from the peripheral blood of patients with ABPA to Asp f I, an 18 kd protein purified from Af extracts. The Asp f I-specific T-cell lines, analyzed by flow cytometry, were 100% CD3+ CD4+. Lymphoproliferative responses of the T-cell lines were specific for Asp f I stimulation, 28,999 cpm (stimulation index = 12.2), and showed no response to tetanus toxoid stimulation, 2178 cpm (stimulation index = 1.1) (p < 0.001). Furthermore, Asp f I-stimulated lymphoproliferation was inhibited in two experiments by monoclonal anti-interleukin (IL)-4 antibody in a dose-response fashion, 78% and 84% inhibition at 5% concentration of anti-IL-4. In contrast, anti-IL-2 antibody did not inhibit Asp f I-stimulated proliferation. Asp f I-stimulated T-cell lines synthesized predominantly IL-4 (mean, 21.5 ng/ml) after 48 hours of culture, and nondetectable quantities of interferon-gamma and IL-2. In summary, Asp f I-specific T-cell lines established from patients with ABPA were characterized as being CD4+ TH2-like in their cytokine synthesis pattern, and secreted IL-4 behaved in an autocrine fashion, stimulating proliferation.
变应性支气管肺曲霉病（ABPA）是一种由烟曲霉（Af）在支气管定植引起的超敏反应性肺部疾病，其特征为血清总IgE水平和Af特异性IgE水平升高以及嗜酸性粒细胞增多。为了检测T细胞对Af抗原的反应性，从ABPA患者的外周血中建立了6个针对从Af提取物中纯化的18kd蛋白Asp f I的T细胞系。通过流式细胞术分析，Asp f I特异性T细胞系100%为CD3 + CD4 +。T细胞系的淋巴细胞增殖反应对Asp f I刺激具有特异性，为28,999 cpm（刺激指数 = 12.2），而对破伤风类毒素刺激无反应，为2178 cpm（刺激指数 = 1.1）（p < 0.001）。此外，在两项实验中，单克隆抗白细胞介素（IL）-4抗体以剂量反应方式抑制了Asp f I刺激的淋巴细胞增殖，在抗IL-4浓度为5%时抑制率分别为78%和84%。相比之下，抗IL-2抗体未抑制Asp f I刺激的增殖。培养48小时后，Asp f I刺激的T细胞系主要合成IL-4（平均21.5 ng/ml），未检测到干扰素-γ和IL-2。总之，从ABPA患者中建立的Asp f I特异性T细胞系在细胞因子合成模式上具有CD4 + TH2样特征，分泌的IL-4以自分泌方式发挥作用，刺激增殖。
