中国南方广州地区变应性支气管肺曲霉病与烟曲霉致敏性哮喘患者中烟曲霉的分子过敏原致敏情况。
Molecular allergen sensitization of Aspergillus fumigatus between allergic bronchopulmonary aspergillosis and A fumigatus-sensitized asthma in Guangzhou, Southern China.
机构信息
Department of Allergy and Clinical Immunology, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Department of Immunology, Guangzhou Kingmed Diagnostics Group Co., Ltd., Guangzhou, China.
出版信息
J Clin Lab Anal. 2020 Oct;34(10):e23448. doi: 10.1002/jcla.23448. Epub 2020 Jul 2.
BACKGROUND
Few studies have assessed the sensitization of mycotic allergens and Aspergillus fumigatus molecular allergens. This study aimed to investigate the relationships of A fumigatus components and mycotic allergens in allergic bronchopulmonary aspergillosis (ABPA) patients and A fumigatus (Af)-sensitized asthma patients.
METHODS
Serum sIgE levels of Penicillium chrysogenum, Cladosporium herbarum, Mucor racemosus, Candida albicans, Alternaria alternata, Helminthosporium halodes, and A fumigatus allergen components (Asp f 1, Asp f 2, Asp f 3, Asp f 4, and Asp f 6) were measured via the ImmunoCAP assay in 18 ABPA and 54 Af-sensitized asthma patients in Guangzhou city, China.
RESULTS
94.44% of ABPA patients and 87.04% of Af-sensitized asthma patients were co-sensitized to at least one other fungal allergen. The positive rates of Asp f 1 (88.89% vs 59.26%, P < .05), Asp f 2 (66.67% vs 33.33%, P < .05), Asp f 4 (61.11% vs 33.33%, P < .05), and Asp f 6 (66.67% vs 14.81%, P < .001) in ABPA patients were higher than those in Af-sensitized asthma patients. IgE levels of Asp f 1 (P < .05), Asp f 4 (P < .05), and Asp f 6 (P < .001) were higher in ABPA patients than in Af-sensitized asthma patients. Optimal scale analysis showed that ABPA was more relevant to Af components (Cronbach's alpha = 90.7%).
CONCLUSION
The A fumigatus components and their relationships with various mycotic allergens were different in ABPA and Af-sensitized asthma patients. This finding may help local doctors in the diagnosis and immunotherapy of fungal allergies.
背景
很少有研究评估过真菌过敏原和烟曲霉分子过敏原的致敏情况。本研究旨在调查烟曲霉成分与变应性支气管肺曲霉病（ABPA）患者和烟曲霉（Af）致敏哮喘患者之间的关系。
方法
采用免疫捕获法检测中国广州 18 例 ABPA 患者和 54 例 Af 致敏哮喘患者血清中对青霉属 Chrysogenum、枝孢属 Herbarum、毛霉属 Racemosus、白色念珠菌、交链孢属 Alternaria、霍氏曲霉 Helminthosporium halodes 和烟曲霉过敏原成分（Asp f 1、Asp f 2、Asp f 3、Asp f 4 和 Asp f 6）的特异性 IgE 水平。
结果
94.44%的 ABPA 患者和 87.04%的 Af 致敏哮喘患者对至少一种其他真菌过敏原呈双阳性。ABPA 患者中 Asp f 1（88.89%比 59.26%，P<0.05）、Asp f 2（66.67%比 33.33%，P<0.05）、Asp f 4（61.11%比 33.33%，P<0.05）和 Asp f 6（66.67%比 14.81%，P<0.001）的阳性率均高于 Af 致敏哮喘患者。ABPA 患者 Asp f 1（P<0.05）、Asp f 4（P<0.05）和 Asp f 6（P<0.001）的 IgE 水平均高于 Af 致敏哮喘患者。最优尺度分析显示，ABPA 与 Af 成分相关性更强（Cronbach's alpha=90.7%）。
结论
ABPA 患者和 Af 致敏哮喘患者的烟曲霉成分及其与各种真菌过敏原的关系存在差异。这一发现可能有助于当地医生对真菌过敏的诊断和免疫治疗。
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