Krisch B
Cell Tissue Res. 1977 Apr 7;179(2):211-24. doi: 10.1007/BF00219797.
Using the immunoenzyme bridge-technique at the light and electron microscopic levels, somatostatin can be demonstrated in the perikarya of the anterior periventricular nucleus, in the median eminence and in the parvocellular hypothalamic nuclei of the rat. In the latter regions the perikarya are negative, whereas a positive reaction for somatostatin is found in a delicate network of fibers and middle-sized granules of very small axons. In light of these results, the double function of somatostatin-as release inhibiting hormone and as transmitter-is discussed. The positive staining reaction in the organum vasculosum laminae terminalis of male and female rats as well as in the subfornical organ, the nucleus dorsalis thalami and the nucleus medialis habenulae in female controls and pregnant rats is not due to somatostatin-containing structures, but partly to substance P and partlly to a substance which could not be defined.
运用免疫酶桥技术，在光学和电子显微镜水平上，可在大鼠室周前核的核周体、正中隆起以及下丘脑小细胞核中证实生长抑素的存在。在后述区域，核周体呈阴性反应，而在纤细的纤维网络和非常小的轴突的中等大小颗粒中发现了生长抑素的阳性反应。鉴于这些结果，对生长抑素作为释放抑制激素和作为神经递质的双重功能进行了讨论。在雄性和雌性大鼠的终板血管器以及雌性对照大鼠和怀孕大鼠的穹窿下器官、丘脑背核和缰核内侧核中的阳性染色反应并非源于含生长抑素的结构，而是部分归因于P物质，部分归因于一种无法确定的物质。
