Ameglio F, Bonifati C, Carducci M, Alemanno L, Sacerdoti G, Fazio M
Laboratory of Clinical Pathology, San Gallicano Institute, Rome, Italy.
Acta Derm Venereol Suppl (Stockh). 1994;186:19-20.
Levels of soluble intercellular adhesion molecule-1 (sICAM-1) and procollagen III peptide (PIIIP) were measured respectively by enzyme immunoassay (EIA) and radioimmunoassay (RIA) methods in sera from 14 patients affected with psoriasis. The same determinations were also performed on suction blister fluids (BFs) obtained from lesional and non-lesional skin. Fourteen normal subjects were used as controls. Significant correlations were found between the serum levels and psoriasis area and severity index (PASI), (R = 0.62 for sICAM-1 and R = 0.73 for PIIIp, respectively). Of the PASI components, infiltration and erythema represented the variables most closely related to PIIIP (R = 0.85; R = 0.72 respectively). Differently from PIIIP, whose levels were significantly lower in the sera than in skin BFs (serum: median value 1.05, range 0.7-2.3 vs. lesional skin fluid: 11.8, 4.8-30 U/ml), sICAM-1 molecules were found predominantly in the sera (serum: median 316, range 117-579 vs lesional skin fluid: median 70, range 31-252 ng/ml). These data cannot exclude that sICAM-1 molecules detected in suction BFs may derive from serum contamination.
采用酶免疫分析（EIA）和放射免疫分析（RIA）方法，分别检测了14例银屑病患者血清中可溶性细胞间黏附分子-1（sICAM-1）和Ⅲ型前胶原肽（PIIIP）的水平。同时，对取自皮损部位和非皮损部位皮肤的水疱抽吸液（BFs）进行了同样的检测。选取14名正常受试者作为对照。发现血清水平与银屑病面积和严重程度指数（PASI）之间存在显著相关性（sICAM-1的R = 0.62，PIIIp的R = 0.73）。在PASI的各组成部分中，浸润和红斑是与PIIIP关系最密切的变量（分别为R = 0.85；R = 0.72）。与PIIIP不同，PIIIP在血清中的水平显著低于皮肤水疱抽吸液（血清：中位数为1.05，范围0.7 - 2.3，而皮损部位皮肤液：11.8，4.8 - 30 U/ml），sICAM-1分子主要存在于血清中（血清：中位数316，范围117 - 579，而皮损部位皮肤液：中位数70，范围31 - 252 ng/ml）。这些数据不能排除在水疱抽吸液中检测到的sICAM-1分子可能来源于血清污染。
