Kennedy B P, Chan C C, Culp S A, Cromlish W A
Department of Molecular Biology, Merck Frosst Centre for Therapeutic Research, Merck Frosst Canada Inc., Pointe Claire-Dorval, Quebec.
Biochem Biophys Res Commun. 1993 Dec 15;197(2):494-500. doi: 10.1006/bbrc.1993.2506.
Rat prostaglandin endoperoxidase synthase-2 (PGHS-2) cDNA was cloned from rat calvarial osteoblasts total RNA by RT-PCR. The primary sequence of rat PGHS-2 had 98% and 92% identity to the mouse and human enzymes, respectively. Transfection of the rat PGHS-2 cDNA into COS 7 cells, followed by the addition of 20 microM arachidonic acid, resulted in a dramatic increase in PGE2 released from these cells. The amount of PGE2 produced was comparable to that obtained from cells similarly transfected with human PGHS-1 cDNA. In the rat paw carrageenin-oedema inflammatory model, the injected paw had elevated levels of PGHS-2 mRNA compared to the control paw. In a rat pyrexia model, injection of the pyrogen lipopolysaccharide, resulted in elevated levels of PGHS-2 mRNA in the brain. These results suggest that PGHS-2 expression plays a role both in inflammation and fever.
通过逆转录聚合酶链反应（RT-PCR）从大鼠颅骨成骨细胞的总RNA中克隆出大鼠前列腺素内过氧化物合酶-2（PGHS-2）的互补DNA（cDNA）。大鼠PGHS-2的一级序列与小鼠和人类的该酶分别具有98%和92%的同源性。将大鼠PGHS-2的cDNA转染到COS 7细胞中，随后添加20微摩尔花生四烯酸，导致这些细胞释放的前列腺素E2（PGE2）显著增加。产生的PGE2量与用人PGHS-1 cDNA进行类似转染的细胞所获得的量相当。在大鼠足跖角叉菜胶性水肿炎症模型中，与对照足相比，注射角叉菜胶的足中PGHS-2信使核糖核酸（mRNA）水平升高。在大鼠发热模型中，注射热原脂多糖导致大脑中PGHS-2 mRNA水平升高。这些结果表明，PGHS-2的表达在炎症和发热中均起作用。
