Habenicht A, Hille S, Knöchel W
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
Biochim Biophys Acta. 1993 Sep 23;1174(3):295-8. doi: 10.1016/0167-4781(93)90202-o.
We have isolated a cDNA clone from a Xenopus laevis tadpole cDNA library which probably codes for the large subunit of glutathione synthetase. The corresponding protein comprises 474 amino acids and shows a significant homology with the large subunit of glutathione synthetase of Schizosaccharomyces pombe. RNase protection experiments revealed that the gene is transcribed during oogenesis and that zygotic expression starts after midblastula transition. Transcripts are also detected in various adult tissues suggesting an ubiquitous distribution of the corresponding protein.
我们从非洲爪蟾蝌蚪的cDNA文库中分离出一个cDNA克隆，它可能编码谷胱甘肽合成酶的大亚基。相应的蛋白质由474个氨基酸组成，与粟酒裂殖酵母谷胱甘肽合成酶的大亚基具有显著的同源性。核糖核酸酶保护实验表明，该基因在卵子发生过程中被转录，合子表达在囊胚中期转变后开始。在各种成年组织中也检测到转录本，这表明相应的蛋白质分布广泛。
