De Veer I, Wilke K, Rüden H
Institut für Hygiene, Freien Universität Berlin.
Zentralbl Hyg Umweltmed. 1994 Jun;195(5-6):516-28.
The bactericide quality of different materials containing copper (copper, brass, brass-sheet) was tested and particularly the effect of humidity and copper-concentration was examined. In first examinations (I. communication) (19) the materials were contaminated by hands, which were dipped into a suspension of different microorganisms (Micrococcus luteus, Staphylococcus aureus, Enterococcus faecium, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa). The survival rate of the microorganisms on the surfaces was determined after defined exposure times (0, 5, 30, 60, 120 min). The following ways of contaminating the test materials were chosen: single contamination with wet hands, repeated contamination with wet and dry hands. In this examination the results were tested for statistical significance and in parallel experiments the copper-concentration on the surfaces of the materials was determined. Materials containing copper contaminated with wet hands show a significant bactericide effect after an exposure time of 120 min. The bactericide effect on Micrococcus luteus and Pseudomonas aeruginosa is inhibited after contamination with dry hands. This results can not be explained by a different copper-concentration on the surfaces of the materials, because a statistically significant difference of copper-ion concentrations between contamination with wet and dry hands could not be obtained.
对不同含铜材料（铜、黄铜、黄铜片）的杀菌质量进行了测试，尤其考察了湿度和铜浓度的影响。在首次试验（第一篇通讯）（19）中，材料通过将手浸入不同微生物（藤黄微球菌、金黄色葡萄球菌、粪肠球菌、大肠杆菌、阴沟肠杆菌、铜绿假单胞菌）的悬浮液中来进行污染。在设定的暴露时间（0、5、30、60、120分钟）后测定微生物在表面的存活率。选择了以下几种污染测试材料的方式：湿手单次污染、湿手和干手重复污染。在本次试验中对结果进行了统计学显著性检验，同时在平行实验中测定了材料表面的铜浓度。湿手污染的含铜材料在暴露120分钟后显示出显著的杀菌效果。干手污染后，对藤黄微球菌和铜绿假单胞菌的杀菌效果受到抑制。该结果无法用材料表面不同的铜浓度来解释，因为湿手污染和干手污染之间铜离子浓度不存在统计学显著差异。
