Austin C, Oka M, Nandha K A, Legon S, Khandan-Nia N, Lo G, Bloom S R
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
J Mol Endocrinol. 1994 Jun;12(3):257-63. doi: 10.1677/jme.0.0120257.
This study has quantified, for the first time, the relative levels of neuromedin U (NmU) mRNA in the rat gastrointestinal tract using Northern blot analysis. NmU message was detected in all regions of the gastrointestinal tract from the oesophagus to the rectum. The greatest levels were found in the duodenum and jejunum, the principal sites for absorption, which were 2.5- and 3-fold respectively above ileal levels. Quantification of NmU mRNA and peptide contents in the duodenum, jejunum and ileum during postnatal development of the rat showed message and peptide levels to be greater in the maturing rat than in neonates. Message levels in the duodenum, jejunum and ileum showed 14-, 7- and 4-fold increases respectively between 1 and 56 days after birth, whilst the corresponding peptide levels in the duodenum, jejunum and ileum showed 33-, 14- and 25-fold increases respectively. Food deprivation caused a small, but significant, decrease in message levels in the jejunum and colon, but there was no change in the duodenum or ileum. This shows that the presence of food has little effect on NmU mRNA levels in the gut.
本研究首次运用Northern印迹分析法对大鼠胃肠道中神经介素U（NmU）mRNA的相对水平进行了定量分析。在从食管到直肠的胃肠道所有区域均检测到了NmU信息。在十二指肠和空肠中发现了最高水平，这两个部位是主要的吸收部位，其水平分别比回肠水平高2.5倍和3倍。对大鼠出生后发育过程中十二指肠、空肠和回肠中NmU mRNA及肽含量的定量分析显示，成熟大鼠中的信息和肽水平高于新生大鼠。出生后1至56天期间，十二指肠、空肠和回肠中的信息水平分别增加了14倍、7倍和4倍，而十二指肠、空肠和回肠中相应的肽水平分别增加了33倍、14倍和25倍。禁食导致空肠和结肠中的信息水平出现小幅但显著的下降，但十二指肠或回肠中没有变化。这表明食物的存在对肠道中NmU mRNA水平影响很小。
