Ouhtit A, Kelly P A, Morel G
Centre National de la Recherche Scientifique Unité de Recherche Associée 1454, Neuroendocrinologie, Faculté de Médecine Lyon-Sud, Oullins, France.
Am J Physiol. 1994 May;266(5 Pt 1):G807-15. doi: 10.1152/ajpgi.1994.266.5.G807.
Several effects of prolactin have been characterized in various tissues of the gastrointestinal tract. In the present study, the expression of short and long forms of prolactin receptor was explored and quantified in the digestive tract and correlated to the prolactin specific functions. Sections of all digestive tissues were analyzed by in situ hybridization, using 35S-labeled oligoprobes unique to each form of receptor. Macroautoradiogram signals were quantified and expressed in arbitrary units. In rat liver, prolactin receptor mRNAs are expressed to a much greater degree in females than in males. The short-form transcript is significantly expressed to a greater degree in liver, whereas the long form predominates in the pancreas and esophagus. In the remainder of the gastrointestinal tract, there is an equivalent distribution of short- and long-form transcripts. Relatively high signal intensities are seen in the stomach, duodenum, jejunum, ileum, and colon, whereas the rectum is essentially negative. The identification of prolactin receptor gene expression to limited regions should help establish specific functions associated with this hormone in the digestive tissues.
催乳素在胃肠道的多种组织中已表现出多种作用。在本研究中，对催乳素受体的短型和长型在消化道中的表达进行了探索和定量分析，并将其与催乳素的特定功能相关联。使用针对每种受体形式的35S标记寡核苷酸探针，通过原位杂交对所有消化组织切片进行分析。对宏观放射自显影片信号进行定量并以任意单位表示。在大鼠肝脏中，催乳素受体mRNA在雌性中的表达程度远高于雄性。短型转录本在肝脏中的表达程度明显更高，而长型则在胰腺和食管中占主导。在胃肠道的其余部分，短型和长型转录本分布相当。在胃、十二指肠、空肠、回肠和结肠中可见相对较高的信号强度，而直肠基本呈阴性。催乳素受体基因在有限区域的表达鉴定应有助于确定该激素在消化组织中的特定功能。
