Tan J, Qiu X F, Xue J L, Zhu X H, Ji X W, Zhang D M, Qin S Z
Genetics Institute, Fudan University, Shanghai, PRC.
Sci China B. 1994 Jun;37(6):701-9.
Using the total human/mouse DNA as the probe, screening has been carried out three times with in situ plaque hybridization to obtain the single-copy DNA sequence from the human X chromosome genomic library. The effective rate of screening is 1.45%. DNAs from clones containing single-copy inserts have been analyzed by a panel of hybrid cells with or without human X chromosome. Three segments, designated by DXFD52,73,75, are mapped to the X chromosome. DXFD52 has been precisely localized on Xq12-q13 with in situ chromosomal hybridization. DXFD52 has been partially sequenced. The results indicate that DXFD52 is a new isolated single-copy segment on the X chromosome. Great progress in the RFLPs study with DXFD52 has been achieved in the population of Chongqing, Sichuan Province. The results show that the DXFD52 can be used to detect the RFLP with Hind III, Bgl II, and Hinf I. DXFD52 will be a potential "landmark" for the construction of the complete linkage map of human genome and the analysis of genomic sequence. And also, the results provide the experience in the development of a useful DNA probe to be applied to diagnosing X-linked disorders.
以人/鼠总DNA为探针，通过原位噬菌斑杂交进行了3次筛选，从人X染色体基因组文库中获得单拷贝DNA序列。筛选有效率为1.45%。用一组含或不含人X染色体的杂交细胞对含单拷贝插入片段的克隆DNA进行了分析。确定了3个片段，命名为DXFD52、73、75，定位于X染色体上。通过染色体原位杂交已将DXFD52精确定位于Xq12 - q13。已对DXFD52进行了部分测序。结果表明DXFD52是X染色体上新分离的单拷贝片段。在四川省重庆人群中，DXFD52的RFLPs研究取得了很大进展。结果表明，DXFD52可用于检测Hind III、Bgl II和Hinf I的RFLP。DXFD52将成为构建人类基因组完整连锁图谱和分析基因组序列的潜在“标记”。此外，这些结果为开发用于诊断X连锁疾病的有用DNA探针提供了经验。
