Zeiller K, Pascher G, Hannig K
Immunology. 1976 Dec;31(6):863-80.
Free-flow electrophoretic separation of mouse spleen cells provides three distinct progenitor cells of direct PFC, showing high, medium and low electrophoretic mobility. All progenitor cells possess surface immunoglobulin and mouse B-lymphocyte specific antigen. The progenitor cells of high electrophoretic mobility show high cycling turnover, a spleen seeking capacity of 16%, provide PFC with a maximum 8 days after transfer and reveal an isometrical increase of the PFC dose response line as a function of the graft size. The progenitor cells of medium electrophoretic mobility are low cycling, 16% home to the spleen, a maximum of PFC is developed eight days after transfer and the PFC dose response line increases allometrically. The progenitor cells of low EPM show low cycling activity, 20% home to the spleen, a maximum of PFC is attained six days after transfer and the PFC dose response line rises isometrically. These results suggest that the electrokinetically different PFC progenitors represent biologically distinct subsets. In double transfer experiments, some evidence was obtained that progenitor cells of low electrophoretic mobility are derived from progenitors of higher electrophoretic mobility. The same observation accounts also for the formation of B lymphocytes of low EPM. Since it seemed likely that the PFC progenitor cells represent virgin cells of a single lineage, the results were discussed in the terms of differentiation pathways of B lymphocytes. A model is considered in which a progenitor of medium electrophoretic mobility provides those of high electrophoretic mobility which after passing a transient cycling stage finally produce mature resting B lymphocytes of low electrophoretic mobility.
对小鼠脾细胞进行自由流动电泳分离可得到三种不同的直接产生抗体形成细胞（PFC）的祖细胞，它们具有高、中、低三种不同的电泳迁移率。所有祖细胞均具有表面免疫球蛋白和小鼠B淋巴细胞特异性抗原。高电泳迁移率的祖细胞具有高循环更新率，归巢至脾脏的能力为16%，转移后最多8天产生PFC，并且PFC剂量反应线随移植物大小呈等比增加。中等电泳迁移率的祖细胞循环率低，16%归巢至脾脏，转移后8天产生最多的PFC，且PFC剂量反应线呈异速增加。低电泳迁移率（EPM）的祖细胞循环活性低，20%归巢至脾脏，转移后6天达到PFC最大值，且PFC剂量反应线呈等比上升。这些结果表明，电泳特性不同的PFC祖细胞代表生物学上不同的亚群。在双重转移实验中，获得了一些证据，表明低电泳迁移率的祖细胞来源于较高电泳迁移率的祖细胞。同样的观察结果也适用于低EPM的B淋巴细胞的形成。由于PFC祖细胞似乎代表单一谱系的原始细胞，因此根据B淋巴细胞的分化途径对结果进行了讨论。提出了一个模型，其中中等电泳迁移率的祖细胞产生高电泳迁移率的祖细胞，后者经过一个短暂的循环阶段后最终产生低电泳迁移率的成熟静止B淋巴细胞。
