Bergqvist B, Arvidsson L, Pettersson E, Galt S, Saalman E, Hamnerius Y, Nordén B
Department of Microwave Technology, Chalmers University of Technology, Gothenburg, Sweden.
Biochim Biophys Acta. 1994 Sep 28;1201(1):51-4. doi: 10.1016/0304-4165(94)90150-3.
The effect of 2.45 GHz microwave radiation on the permeability of unilamellar phosphatidylcholine liposomes has been studied. Leakage of 5(6)-carboxyfluorescein from the liposomes was measured using spectrofluorimetry after exposure to either microwaves or thermal heating for 5-20 min intervals. The exposure temperature, 37.6 +/- 0.5 degrees C, was well above the phase transition temperature of the lipid membrane. The microwave exposure did not result in any non-thermal increase in permeability above that produced by thermal heating. This study refutes the results reported by Saalman et al. [1] in which an increased liposome permeability due to microwave exposure was reported. The refined analysis in the present study shows that this increased liposome permeability was not a non-thermal microwave effect.
研究了2.45 GHz微波辐射对单层磷脂酰胆碱脂质体通透性的影响。在以5 - 20分钟为间隔暴露于微波或热加热后，使用荧光分光光度法测量脂质体中5(6)-羧基荧光素的泄漏情况。暴露温度为37.6±0.5℃，远高于脂质膜的相变温度。微波暴露并未导致通透性出现高于热加热所产生的任何非热增加。本研究反驳了萨尔曼等人[1]报道的结果，他们报告称微波暴露会导致脂质体通透性增加。本研究的精细分析表明，这种脂质体通透性增加并非非热微波效应。
