Saalman E, Nordén B, Arvidsson L, Hamnerius Y, Höjevik P, Connell K E, Kurucsev T
Department of Physical Chemistry, Chalmers University of Technology, Gothenburg, Sweden.
Biochim Biophys Acta. 1991 Apr 26;1064(1):124-30. doi: 10.1016/0005-2736(91)90418-8.
The influence of 2.45 GHz microwave radiation on the membrane permeability of unilamellar liposomes was studied using the marker 5(6)-carboxyfluorescein trapped in phosphatidylcholine liposomes. The release of the fluorescent marker was followed by spectrofluorimetry after an exposure of 10 minutes to either microwave radiation or to heat alone of the liposome solutions. A significant increase of the permeability of carboxyfluorescein through the membrane was observed for the microwave-exposed samples compared to those exposed to normal heating only. Exposure to 2.45 GHz microwave radiation of liposomes has been previously found to produce increased membrane permeability as compared with heating. However, in contrast to previous studies, the observations reported here were made above the phase transition temperature of the lipid membrane. The experimental setup included monitoring of the temperature during microwave exposure simultaneously at several points in the solution volume using a fiberoptic thermometer. Possible mechanisms to explain the observations are discussed.
利用包裹在磷脂酰胆碱脂质体中的5(6)-羧基荧光素作为标记物，研究了2.45 GHz微波辐射对单层脂质体膜通透性的影响。在脂质体溶液暴露于微波辐射或仅加热10分钟后，通过荧光分光光度法跟踪荧光标记物的释放。与仅暴露于正常加热的样品相比，观察到微波暴露样品中羧基荧光素透过膜的通透性显著增加。与加热相比，先前已发现脂质体暴露于2.45 GHz微波辐射会导致膜通透性增加。然而，与先前的研究不同，此处报告的观察结果是在脂质膜的相变温度以上进行的。实验装置包括使用光纤温度计在溶液体积的几个点同时监测微波暴露期间的温度。讨论了解释这些观察结果的可能机制。
