Orlando A R, Mossa G, D'Inzeo G
Institute of Experimental Medicine, CNR, Rome, Italy.
Bioelectromagnetics. 1994;15(4):303-13. doi: 10.1002/bem.2250150405.
The influence of 2.45 GHz microwave exposure (6 mW/g) on the diffusion processes in enzyme-loaded unilamellar liposomes as bioreactors was studied. The enzyme carbonic anhydrase (CA) was entrapped into cationic unilamellar vesicles. Previous kinetic experiments showed a very low self-diffusion rate of the substrate p-nitrophenyl acetate (PNPA) across intact liposome bilayer. A twofold increase in the diffusion rate of PNPA through the lipid bilayer was observed after 120 min of microwave radiation compared to temperature control samples. The microwave effect was time dependent. The enzyme activity, as a function of increased diffusion of PNPA, rises over 120 min from 22.3% to 80%. The increase in stearylamine concentration reduces the enzyme activity from 80% to 65% at 120 min. No enzyme leakage was observed.
研究了2.45 GHz微波辐射（6 mW/g）对作为生物反应器的载酶单层脂质体中扩散过程的影响。将碳酸酐酶（CA）包封到阳离子单层囊泡中。先前的动力学实验表明，底物对硝基苯乙酸（PNPA）在完整脂质体双层中的自扩散速率非常低。与温度对照样品相比，微波辐射120分钟后，观察到PNPA通过脂质双层的扩散速率增加了两倍。微波效应是时间依赖性的。作为PNPA扩散增加的函数，酶活性在120分钟内从22.3%上升到80%。硬脂胺浓度的增加在120分钟时将酶活性从80%降低到65%。未观察到酶泄漏。
