Mailleux P, Verslype M, Preud'homme X, Vanderhaeghen J J
Unité de Recherche sur le Cerveau, Faculté de Médecine, Université Libre de Bruxelles, Belgium.
Neuroreport. 1994 Jun 2;5(10):1265-8. doi: 10.1097/00001756-199406020-00028.
delta-9-Tetrahydrocannabinol (THC) is a major psychoactive component of cannabis. We have recently localized a receptor for THC in the forebrain and found in the caudate-putamen that its gene expression is modulated by glucocorticoids, dopamine and glutamate. Here, we report for the first time, using quantitative in situ hybridization, that acute THC (5 mg kg-1, i.p.) regulates the mRNA levels of multiple immediate early genes in the adult rat forebrain. Twenty minutes after a single THC injection, significant increases in concentration of the mRNAs for C-FOS, C-JUN and ZIF-268 were observed in the cingulate cortex (75, 45 and 37%) and for C-FOS and ZIF-268 in the fronto-parietal cortex (60 and 64%) and caudate-putamen (81 and 32%) while JUN-D mRNA levels were not changed. These transcription factor genes might mediate putative THC modulation of neurotransmitter gene expression.
Δ⁹-四氢大麻酚（THC）是大麻的主要精神活性成分。我们最近在前脑定位了一种THC受体，并在尾状核-壳核中发现其基因表达受糖皮质激素、多巴胺和谷氨酸调节。在此，我们首次使用定量原位杂交技术报告，急性给予THC（5毫克/千克，腹腔注射）可调节成年大鼠前脑中多个即刻早期基因的mRNA水平。单次注射THC 20分钟后，在扣带回皮质中观察到C-FOS、C-JUN和ZIF-268的mRNA浓度显著增加（分别为75%、45%和37%），在额顶叶皮质中C-FOS和ZIF-268的mRNA浓度显著增加（分别为60%和64%），在尾状核-壳核中C-FOS和ZIF-268的mRNA浓度显著增加（分别为81%和32%），而JUN-D的mRNA水平未发生变化。这些转录因子基因可能介导THC对神经递质基因表达的假定调节作用。
