Niclou S, Suidan H S, Brown-Luedi M, Monard D
Friedrich Miescher-Institut, Basel, Switzerland.
Cell Mol Biol (Noisy-le-grand). 1994 May;40(3):421-8.
Thrombin activates its receptor in a number of cultured cells of neural origin, but the functional significance of this activation in the nervous system is unknown. It is also not known which cells in brain express the thrombin receptor and whether the level of its expression is developmentally regulated. In the present study, Northern blot analysis showed that thrombin receptor mRNA was expressed at higher levels in brain compared to some other tissues, such as skeletal muscle, liver or kidney. The level of expression is substantially higher in the brain of newborn rats compared to that of postnatal day 28 (P28). At embryonic day 18, thrombin receptor mRNA is present throughout in the brain and in dorsal root ganglia as detected by in situ hybridization. The regions of the P28 brain in which the thrombin receptor mRNA was present include the substantia nigra and the ventral tegmental area, the pretectal area, some hypothalamic nuclei and some cells of the cerebral cortex. These results represent one of the first steps needed to understand the role played by the thrombin receptor in the development and function of the nervous system.
凝血酶可激活多种神经源性培养细胞中的受体，但其在神经系统中激活作用的功能意义尚不清楚。同样未知的是，脑中哪些细胞表达凝血酶受体，以及其表达水平是否受发育调控。在本研究中，Northern印迹分析显示，与骨骼肌、肝脏或肾脏等其他一些组织相比，凝血酶受体mRNA在脑中的表达水平更高。新生大鼠脑内的表达水平显著高于出生后第28天（P28）。原位杂交检测显示，在胚胎第18天，凝血酶受体mRNA遍布于脑和背根神经节。P28脑内存在凝血酶受体mRNA的区域包括黑质、腹侧被盖区、顶盖前区、一些下丘脑核以及大脑皮质的一些细胞。这些结果是理解凝血酶受体在神经系统发育和功能中所起作用所需的最初步骤之一。
