采用液相色谱-串联质谱法和稳定同位素稀释分析法对食品、尿液和血浆中的胡芦巴碱、烟酸和烟酰胺进行定量研究。
Quantitative investigation of trigonelline, nicotinic acid, and nicotinamide in foods, urine, and plasma by means of LC-MS/MS and stable isotope dilution analysis.
作者信息
Lang Roman, Yagar Erkan Firat, Eggers Rudolf, Hofmann Thomas
机构信息
Technische Universitat Munchen, Freising, Germany.
出版信息
J Agric Food Chem. 2008 Dec 10;56(23):11114-21. doi: 10.1021/jf802838s.
A straightforward stable isotope dilution analysis (SIDA) for the quantitative determination of trigonelline, nicotinic acid, and nicotinamide in foods such as coffee, as well as in biological samples by means of LC-MS/MS (MRM) has been developed. The coefficients of variation for their quantitative analysis in a coffee sample were 2.1% for trigonelline, 1.1% for nicotinic acid, and 3.1% for nicotinamide, and recovery experiments showed good results between 98.5 and 104.5%. Application of this SIDA for the quantification of trigonelline, nicotinic acid, and nicotinamide in coffee samples of different roasting degrees revealed a drastic degradation of trigonelline as well as the generation of nicotinic acid accounting for 4-6% of the initial trigonelline content, whereas nicotinamide remained rather constant at a low level. Besides the analysis of coffee samples, the feasibility of the developed SIDA was verified by analysis of other foods including breakfast cereals, rice, liver, and herring, as well as human urine and plasma samples.
已开发出一种直接稳定同位素稀释分析（SIDA）方法，用于通过液相色谱 - 串联质谱法（MRM）定量测定咖啡等食品以及生物样品中的胡芦巴碱、烟酸和烟酰胺。在咖啡样品中对它们进行定量分析时，胡芦巴碱的变异系数为2.1%，烟酸为1.1%，烟酰胺为3.1%，回收率实验结果良好，在98.5%至104.5%之间。将这种SIDA应用于不同烘焙程度咖啡样品中胡芦巴碱、烟酸和烟酰胺的定量分析，结果表明胡芦巴碱急剧降解，同时生成了占初始胡芦巴碱含量4 - 6%的烟酸，而烟酰胺在低水平上保持相当稳定。除了分析咖啡样品外，还通过分析其他食品（包括早餐谷物、大米、肝脏和鲱鱼）以及人体尿液和血浆样品，验证了所开发的SIDA的可行性。
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