Robison K, Gilbert W, Church G M
Department of Cellular and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Nat Genet. 1994 Jun;7(2):205-14. doi: 10.1038/ng0694-205.
DNA sequencing efforts frequently uncover genes other than the targeted ones. We have used rapid database scanning methods to search for undescribed eubacterial and archean protein coding frames in regions flanking known genes. By searching all prokaryotic DNA sequences not marked as coding for proteins or stable RNAs against the protein databases, we have identified more than 450 new examples of bacterial proteins, as well as a smaller number of possible revisions to known proteins, at a surprisingly high rate of one new protein or revision for every 24 initial DNA sequences or 8,300 nucleotides examined. Seven proteins are members of families which have not been described in prokaryotic sequences. We also describe 49 re-interpretations of existing sequence data of particular biological significance.
DNA测序工作经常会发现目标基因以外的其他基因。我们利用快速数据库扫描方法，在已知基因侧翼区域搜索未描述的真细菌和古细菌蛋白质编码框架。通过针对蛋白质数据库搜索所有未标记为编码蛋白质或稳定RNA的原核DNA序列，我们已经以惊人的高速度识别出超过450个细菌蛋白质新实例，以及对已知蛋白质的少量可能修正，即每检查24个初始DNA序列或8300个核苷酸就有一个新蛋白质或修正。七种蛋白质属于原核序列中未描述的家族成员。我们还描述了49个具有特殊生物学意义的现有序列数据的重新解读。
