de Messias I T, Mohren D
Department of Clinical Pathology, University Hospital, UFPR, Curitiba, Brazil.
J Investig Allergol Clin Immunol. 1994 Mar-Apr;4(2):91-5.
The following study presents evidence of complement activation in paracoccidioidomycosis (PCM). Twenty-eight untreated patients were studied from endemic areas of Parana in southern Brazil. The activation of the classical pathway, evaluated by the C4d/C4 ratio, was significantly elevated in the patients compared to the control population (p < 0.005). Six patients were examined prospectively with the C4d/C4 assay during treatment and they showed a decrease in this ratio associated with clinical improvement. The activation of the alternative pathway was determined by rocket immunoelectrophoresis of fragment Ba. These levels were also significantly higher in the patient group in comparison to the controls (p < 0.0005). The prospective study also showed a significant variation in the Ba levels associated with clinical improvement (p < 0.01). Furthermore, the levels of C3, C4, CH50 and anti-Paracoccidioides brasiliensis IgG were determined in all patients. The anti-P. brasiliensis IgG levels showed a weak positive correlation with the C4d/C4 ratio (r[S] = 0.45; p < 0.03). The C3, C4 and CH50 levels did not show significant variations from the normal ranges. Our results suggest the involvement of both complement pathways, classical and alternative, in PCM and their association with disease activity.
以下研究展示了副球孢子菌病（PCM）中补体激活的证据。对来自巴西南部巴拉那州流行地区的28名未经治疗的患者进行了研究。通过C4d/C4比值评估的经典途径激活在患者中显著高于对照人群（p < 0.005）。在治疗期间对6名患者进行了C4d/C4检测的前瞻性研究，结果显示该比值下降且临床症状改善。通过Ba片段的火箭免疫电泳测定替代途径的激活情况。与对照组相比，患者组中这些水平也显著更高（p < 0.0005）。前瞻性研究还显示Ba水平与临床改善存在显著差异（p < 0.01）。此外，测定了所有患者的C3、C4、CH50和抗巴西副球孢子菌IgG水平。抗巴西副球孢子菌IgG水平与C4d/C4比值呈弱正相关（r[S] = 0.45；p < 0.03）。C3、C4和CH50水平与正常范围无显著差异。我们的结果表明经典途径和替代途径均参与了PCM，且它们与疾病活动相关。
