Cos S, Sánchez-Barceló E J
Department of Physiology and Pharmacology, School of Medicine, University of Cantabria, Santander, Spain.
Cancer Lett. 1994 Sep 30;85(1):105-9. doi: 10.1016/0304-3835(94)90245-3.
We studied the different in vitro antiproliferative actions of melatonin on MCF-7 cells, depending on whether the cells are exposed to hormone concentrations which remain constant in culture media (Group I, 10(-9) M; Group II, 10(-11) M melatonin) or varying at 12 h intervals, thus simulating a diurnal rhythm: Group III, 12 h in 10(-9) M melatonin/12 h without melatonin (10(-9) M/0 12/12 h); Group IV, 10(-11) M/0 12/12 h; Group V, 10(-9) M/10(-11) M 12/12 h. After 5 days of culture, cell proliferation appeared significantly inhibited in Groups I and III, but not in Groups II and IV. However, the highest antiproliferative effect was obtained by sequential exposure to 10(-9) M/10(-11) M melatonin (Group V), which mimics the physiological rhythm of serum melatonin concentration.
我们研究了褪黑素对MCF - 7细胞不同的体外抗增殖作用，这取决于细胞是暴露于在培养基中保持恒定的激素浓度（第一组，10(-9) M；第二组，10(-11) M褪黑素），还是以12小时间隔变化，从而模拟昼夜节律：第三组，12小时暴露于10(-9) M褪黑素/12小时无褪黑素（10(-9) M/0 12/12小时）；第四组，10(-11) M/0 12/12小时；第五组，10(-9) M/10(-11) M 12/12小时。培养5天后，第一组和第三组细胞增殖明显受到抑制，而第二组和第四组则没有。然而，通过依次暴露于10(-9) M/10(-11) M褪黑素（第五组）获得了最高的抗增殖效果，该组模拟了血清褪黑素浓度的生理节律。
