Wernert N, Gilles F, Fafeur V, Bouali F, Raes M B, Pyke C, Dupressoir T, Seitz G, Vandenbunder B, Stéhelin D
CNRS URA 1160, Institut Pasteur, Lille, France.
Cancer Res. 1994 Nov 1;54(21):5683-8.
The stroma reaction has an important role in tumor growth, invasion, and metastasis. In various invasive human carcinomas, as well as in a mouse model for tumor invasion, transcripts encoding the transcription factor c-Ets1 were detected within stromal fibroblasts, whereas they were absent in epithelial tumor cells. This expression of c-Ets1 was often increased in fibroblasts directly adjacent to neoplastic cells. Endothelial cells of stromal capillaries were also positive for c-Ets1 expression. In contrast, fibroblasts of corresponding noninvasive lesions and of normal tissues were consistently negative. In cultured human fibroblasts stimulated by basic fibroblast growth factor and tumor necrosis factor alpha, the expression of c-Ets1 correlated with the accumulation of transcripts for potential target genes, collagenase-1 and stromelysin-1. The same correlation was observed in some of the invasive carcinomas investigated. These results suggest that c-Ets1 participates in the regulation of tumor invasion in vivo.
基质反应在肿瘤生长、侵袭和转移中具有重要作用。在各种侵袭性人类癌组织以及肿瘤侵袭的小鼠模型中，编码转录因子c-Ets1的转录本在基质成纤维细胞中被检测到，而上皮肿瘤细胞中则不存在。c-Ets1的这种表达在紧邻肿瘤细胞的成纤维细胞中常常增加。基质毛细血管的内皮细胞c-Ets1表达也呈阳性。相比之下，相应的非侵袭性病变和正常组织中的成纤维细胞始终呈阴性。在受碱性成纤维细胞生长因子和肿瘤坏死因子α刺激的培养人成纤维细胞中，c-Ets1的表达与潜在靶基因胶原酶-1和基质溶解素-1转录本的积累相关。在一些所研究的侵袭性癌组织中也观察到了同样的相关性。这些结果表明，c-Ets1参与体内肿瘤侵袭的调控。
