Tohjima T, Arisaka T, Honda N
Department of Internal Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Clin Ther. 1994 May-Jun;16(3):405-15.
The effects of trapidil, a coronary vasodilator and platelet aggregation inhibitor, on fatty acid metabolism and prostaglandin (PG) formation in platelets were studied using platelet suspensions from six normal subjects. The addition of trapidil to fatty acids in platelet phospholipids decreased palmitoleic acid and arachidonic acid, and increased an unidentified substance, X2 (palmitoleic acid, P < 0.05; arachidonic acid, P < 0.05; X2, P < 0.05). Thrombin stimulation following the addition of trapidil resulted in an increase in stearic acid and a decrease in arachidonic acid, compared with the trapidil-free control samples (stearic acid, P < 0.05; arachidonic acid, P < 0.02). The addition of trapidil tended to increase immunoreactive PGE (iPGE) and iPGF dose-dependently. On the other hand, thrombin stimulation following the addition of trapidil decreased the formation of thromboxane B2 (TXB2) significantly compared with the levels of TXB2 in the trapidil-free samples (10 micrograms/mL trapidil, P < 0.005, 100 micrograms/mL trapidil, P < 0.001). These results show that trapidil increased arachidonic acid mobilization in the platelets.
使用六名正常受试者的血小板悬液，研究了冠脉血管扩张剂和血小板聚集抑制剂曲匹地尔对血小板脂肪酸代谢和前列腺素（PG）生成的影响。向血小板磷脂中的脂肪酸添加曲匹地尔可降低棕榈油酸和花生四烯酸，并增加一种未鉴定物质X2（棕榈油酸，P<0.05；花生四烯酸，P<0.05；X2，P<0.05）。与未添加曲匹地尔的对照样品相比，添加曲匹地尔后的凝血酶刺激导致硬脂酸增加，花生四烯酸减少（硬脂酸，P<0.05；花生四烯酸，P<0.02）。添加曲匹地尔倾向于剂量依赖性地增加免疫反应性PGE（iPGE）和iPGF。另一方面，与未添加曲匹地尔样品中的血栓素B2（TXB2）水平相比，添加曲匹地尔后的凝血酶刺激显著降低了TXB2的生成（10μg/mL曲匹地尔，P<0.005，100μg/mL曲匹地尔，P<0.001）。这些结果表明曲匹地尔增加了血小板中花生四烯酸的动员。
