Mindrinos M, Katagiri F, Yu G L, Ausubel F M
Department of Genetics, Harvard Medical School, Boston, Massachusetts.
Cell. 1994 Sep 23;78(6):1089-99. doi: 10.1016/0092-8674(94)90282-8.
In plants, resistance to a pathogen is frequently correlated with a genetically defined interaction between a plant resistance gene and a corresponding pathogen avirulence gene. A simple model explains these gene-for-gene interactions: avirulence gene products generate signals (ligands), and resistance genes encode cognate receptors. The A. thaliana RPS2 gene confers resistance to the bacterial pathogen P. syringae carrying the avirulence gene avrRpt2. A map-based positional cloning strategy was used to identify RPS2. The identification of RPS2 was verified using a newly developed transient assay for RPS2 function and by genetic complementation in transgenic plants. RPS2 encodes a novel 105 kDa protein containing a leucine zipper, a nucleotide-binding site, and 14 imperfect leucine-rich repeats.
在植物中，对病原体的抗性通常与植物抗性基因和相应病原体无毒基因之间的遗传定义相互作用相关。一个简单的模型解释了这些基因对基因的相互作用：无毒基因产物产生信号（配体），而抗性基因编码同源受体。拟南芥RPS2基因赋予对携带无毒基因avrRpt2的细菌病原体丁香假单胞菌的抗性。采用基于图谱的定位克隆策略来鉴定RPS2。使用新开发的RPS2功能瞬时检测法和转基因植物中的遗传互补法验证了RPS2的鉴定。RPS2编码一种新型的105 kDa蛋白质，该蛋白质含有一个亮氨酸拉链、一个核苷酸结合位点和14个不完全的富含亮氨酸重复序列。
