Lawrence G J, Finnegan E J, Ayliffe M A, Ellis J G
Commonwealth Scientific and Industrial Research Organisation, Division of Plant Industry, Canberra, Australia.
Plant Cell. 1995 Aug;7(8):1195-206. doi: 10.1105/tpc.7.8.1195.
The L6 rust resistance gene from flax was cloned after tagging with the maize transposable element Activator. The gene is predicted to encode two products of 1294 and 705 amino acids that result from alternatively spliced transcripts. The longer product is similar to the products of two other plant disease resistance genes, the tobacco mosaic virus resistance gene N of tobacco and the bacterial resistance gene RPS2 of Arabidopsis. The similarity involves the presence of a nucleotide (ATP/GTP) binding site and several other amino acid motifs of unknown function in the N-terminal half of the polypeptides and a leucine-rich region in the C-terminal half. The truncated product of L6, which lacks most of the leucine-rich C-terminal region, is similar to the truncated product that is predicted from an alternative transcript of the N gene. The L6, N, and RPS2 genes, which control resistance to three widely different pathogen types, are the foundation of a class of plant disease resistance genes that can be referred to as nucleotide binding site/leucine-rich repeat resistance genes.
利用玉米转座因子激活子对亚麻的L6抗锈病基因进行标记后，该基因被克隆出来。预测该基因编码两种由可变剪接转录本产生的产物，分别含1294个和705个氨基酸。较长的产物与另外两个植物抗病基因的产物相似，即烟草的烟草花叶病毒抗性基因N和拟南芥的细菌抗性基因RPS2。这种相似性包括在多肽的N端一半区域存在一个核苷酸（ATP/GTP）结合位点和几个功能未知的其他氨基酸基序，以及在C端一半区域存在一个富含亮氨酸的区域。L6的截短产物缺少大部分富含亮氨酸的C端区域，与从N基因的可变转录本预测的截短产物相似。L6、N和RPS2基因控制对三种广泛不同病原体类型的抗性，它们是一类植物抗病基因的基础，这类基因可被称为核苷酸结合位点/富含亮氨酸重复序列抗性基因。
