Nowack H, Gay S, Wick G, Becker U, Timpl R
J Immunol Methods. 1976;12(1-2):117-24. doi: 10.1016/0022-1759(76)90101-0.
Antibodies to bovine type I and type III collagen and their precursor form procollagen were produced in rabbits and rendered specific for the immunizing antigen by immunoadsorption. These purified antibodies showed distinct immunofluorescence staining on frozen sections of both bovine and human connective tissue at concentrations as low as 1-10 mug/ml. Antibodies to type III collagen and procollagen reacted with reticulin in liver and spleen, with fascicles around tendons and with the upper portion of the dermis. Antibodies to type I collagen and procollagen reacted with skin and fiber bundles in tendon but did not stain reticulin. No reaction was observed with cartilage collagen or with kidney glomerular basement membrane.
针对牛I型和III型胶原蛋白及其前体形式原胶原蛋白的抗体在兔体内产生，并通过免疫吸附使其对免疫抗原具有特异性。这些纯化的抗体在浓度低至1-10微克/毫升时，对牛和人结缔组织的冰冻切片显示出明显的免疫荧光染色。III型胶原蛋白和原胶原蛋白的抗体与肝脏和脾脏中的网状纤维、肌腱周围的束状结构以及真皮上部发生反应。I型胶原蛋白和原胶原蛋白的抗体与皮肤和肌腱中的纤维束发生反应，但不使网状纤维染色。未观察到与软骨胶原蛋白或肾小球基底膜的反应。
