Hoffman R M
AntiCancer, Inc., San Diego, CA 92111.
Cancer Metastasis Rev. 1994 Jun;13(2):169-73. doi: 10.1007/BF00689634.
In vivo-like drug responses are observed in three-dimensional culture but frequently not in two-dimensional culture, indicating that drug response may be a function of tissue architecture. Alexis Carrel introduced that in vitro culture of tissues in the beginning of the century utilizing a culture system that allowed the three-dimensional growth of tissues. Leighton improved upon this system by developing a substrate of sponge matrices. Other methods of three-dimensional culture include collagen gels and what are known as organ culture systems on filters or meshes. In addition, cell suspensions can be converted into multicellular spheroids, another form of three-dimensional culture. Comparison of the three-dimensional culture methods with two-dimensional culture methods has shown critical differences in drug response. The in vivo mechanism of drug resistance may involve alterations in cell-cell interaction which may occur in three-dimensional culture as opposed to monolayer culture.
在三维培养中可观察到类似体内的药物反应，但在二维培养中通常观察不到，这表明药物反应可能是组织结构的一种功能。亚历克西斯·卡雷尔在本世纪初引入了利用允许组织三维生长的培养系统进行组织体外培养。莱顿通过开发海绵基质底物改进了该系统。三维培养的其他方法包括胶原凝胶以及在滤器或网格上的所谓器官培养系统。此外，细胞悬液可转化为多细胞球体，这是三维培养的另一种形式。三维培养方法与二维培养方法的比较显示出药物反应的关键差异。体内耐药机制可能涉及细胞间相互作用的改变，这种改变可能发生在三维培养而非单层培养中。
