Bachorik P S, Lovejoy K L, Carroll M D, Johnson C L, Albers J J, Marcovina S M
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287.
Clin Chem. 1994 Oct;40(10):1915-20.
We measured apolipoproteins (apo) A-I and B by rate immunonephelometry (rate INA) during Phase 1 of the National Health and Nutrition Examination Survey (NHANES) III. We also made the measurements by radial immunodiffusion (RID) in a 20% subset of the samples. Aliquots of this subset were also analyzed in the Northwest Lipid Research Laboratories by fixed-time INA calibrated to the World Health Organization (WHO)-International Federation of Clinical Chemistry (IFCC) First International Reference Materials for Apolipoproteins A-I and B. The CVs for the rate INA and RID measurements were: apoA-I, 4.5-7.7% and 2.5-7.6%, respectively; apoB, 2.3-5.3% and 2.3-6.4%, respectively. In NHANES III, rate INA values (x) can be transformed to WHO-IFCC Reference Material-based values (y) as follows: for apoA-I, y = 0.87x + 251.8 mg/L (r = 0.93, SEslope = 0.13, SEintercept = 17, n = 708); for apoB (mg/L), y = 1.068x + 112.8 mg/L (r = 0.98, SEslope = 0.08, SEintercept = 7, n = 646).
在第三次全国健康与营养检查调查（NHANES III）的第一阶段，我们通过速率免疫比浊法（速率INA）测量了载脂蛋白（apo）A-I和B。我们还在20%的样本子集中通过放射免疫扩散法（RID）进行了测量。该子集的等分试样也在西北脂质研究实验室通过固定时间INA进行分析，该方法已根据世界卫生组织（WHO）-国际临床化学联合会（IFCC）载脂蛋白A-I和B的第一国际参考物质进行校准。速率INA和RID测量的变异系数分别为：apoA-I分别为4.5 - 7.7%和2.5 - 7.6%；apoB分别为2.3 - 5.3%和2.3 - 6.4%。在NHANES III中，速率INA值（x）可以按照以下方式转换为基于WHO-IFCC参考物质的值（y）：对于apoA-I，y = 0.87x + 251.8 mg/L（r = 0.93，斜率标准误 = 0.13，截距标准误 = 17，n = 708）；对于apoB（mg/L），y = 1.068x + 112.8 mg/L（r = 0.98，斜率标准误 = 0.08，截距标准误 = 7，n = 646）。
