Jones-Mortimer M C, Kornberg H L
J Gen Microbiol. 1976 Oct;96(2):383-91. doi: 10.1099/00221287-96-2-383.
Strains of Escherichia coli that are unable to grow on fructose because they lack the phosphoenolpyruvate: fructose phosphotransferases specified by ptsF and ptsX mutate to grow on media containing fructose as sole carbon source, but do not regain the function of either of the missing phosphotransferases. Instead, fructose is taken up and phosphorylated to fructose 6-phosphate by a phosphoenolpyruvate: sorbitol phosphotransferase which, in wild-type cells, is induced by sorbitol but not by fructose, but which is constitutively expressed in these mutants. The regulatory gene srlC controlling enzymes of sorbitol uptake and catabolism has been located on the E. coli genome as part of the linkage group cysI srlC attI86 pheA.
由于缺乏由ptsF和ptsX指定的磷酸烯醇丙酮酸：果糖磷酸转移酶而无法在果糖上生长的大肠杆菌菌株会发生突变，从而能够在以果糖作为唯一碳源的培养基上生长，但不会恢复任何一种缺失的磷酸转移酶的功能。相反，果糖通过磷酸烯醇丙酮酸：山梨醇磷酸转移酶被摄取并磷酸化为6-磷酸果糖，在野生型细胞中，该酶由山梨醇诱导而非果糖诱导，但在这些突变体中组成性表达。控制山梨醇摄取和分解代谢酶的调控基因srlC已定位在大肠杆菌基因组上，作为连锁群cysI srlC attI86 pheA的一部分。
