Qadri F, Azim T, Hossain A, Chowdhury A, Albert M J
International Centre for Diarrhoeal Disease Research-Bangladesh, Dhaka.
Diagn Microbiol Infect Dis. 1994 Mar;18(3):145-9. doi: 10.1016/0732-8893(94)90083-3.
Three mouse monoclonal antibodies (mAbs) (ICL3, ICL4, and ICL5) were produced that specifically recognized the lipopolysaccharide antigen of the newly recognized Shigella dysenteriae serotype-13 strain. All three mAbs reacted with all nine reference isolates of S. dysenteriae 13 in different tests. The mAbs also detected colonies of S. dysenteriae-13 isolates by direct slide agglutination test. The mAbs also reacted with the reference Escherichia coli 0150 strain and showed its close antigenic relationship with S. dysenteriae 13. Use of these mAbs in our clinical laboratory during an 8-month period detected three S. dysenteriae-13 isolates that were also detected by a polyclonal rabbit antiserum. It should now be possible to define the epidemiologic importance of S. dysenteriae serotype 13 in diarrhea by using these mAbs.
制备了三种小鼠单克隆抗体（mAb）（ICL3、ICL4和ICL5），它们能特异性识别新确认的痢疾志贺氏菌血清型13菌株的脂多糖抗原。在不同试验中，这三种单克隆抗体均与痢疾志贺氏菌13的所有九株参考分离株发生反应。这些单克隆抗体还通过直接玻片凝集试验检测到痢疾志贺氏菌13分离株的菌落。这些单克隆抗体也与参考大肠杆菌0150菌株发生反应，显示出其与痢疾志贺氏菌13密切的抗原关系。在我们的临床实验室中，在8个月的时间里使用这些单克隆抗体检测到三株痢疾志贺氏菌13分离株，多克隆兔抗血清也检测到了这些分离株。现在应该可以通过使用这些单克隆抗体来确定痢疾志贺氏菌血清型13在腹泻中的流行病学重要性。
