de Silva D G, Candy D C, Mendis L N, Chart H, Rowe B
Department of Child Health, King's College School of Medicine and Dentistry, London, U.K.
J Infect. 1992 Nov;25(3):273-8. doi: 10.1016/0163-4453(92)91539-n.
A total of 192 samples of serum from 113 Sri Lankan patients with clinical dysentery was examined for antibodies of the IgM class to the lipopolysaccharides (LPSs) of Shigella dysenteriae-1 and Escherichia coli O157:H7. By means of ELISA and immunoblotting, 59 patients were found to have serum antibodies to the LPS of S. dysenteriae-1 only. Four samples from one patient were found to contain serum antibodies to the LPSs of both S. dysenteriae-1 and E. coli O157:H7. Antibodies to the LPS of S. dysenteriae-1 were also detected in 16 samples from 25 children, from Sri Lanka, with no previous history of dysentery; one of these children also had antibodies to the LPS of E. coli O157:H7. Analysis of 16 samples from apparently healthy children in the U.K. showed that only one serum contained antibodies to the LPS of S. dysenteriae-1. This patient had a history of recent travel to Pakistan. The isolation of S. dysenteriae-1 remains the preferred test for the diagnosis of bacillary dysentery. The use of serology as a means of providing evidence of infection with S. dysenteriae-1, however may prove to be a useful adjunct to cultural techniques but needs to be validated in an area where this organism is endemic.
对113名患有临床痢疾的斯里兰卡患者的总共192份血清样本进行检测，以查找针对痢疾志贺氏菌1型和大肠杆菌O157:H7脂多糖（LPS）的IgM类抗体。通过酶联免疫吸附测定（ELISA）和免疫印迹法，发现59名患者仅对痢疾志贺氏菌1型的LPS有血清抗体。从一名患者的四份样本中发现含有针对痢疾志贺氏菌1型和大肠杆菌O157:H7的LPS的血清抗体。在来自斯里兰卡的25名无痢疾既往史的儿童的16份样本中也检测到了针对痢疾志贺氏菌1型LPS的抗体；其中一名儿童还对大肠杆菌O157:H7的LPS有抗体。对英国表面健康儿童的16份样本进行分析表明，只有一份血清含有针对痢疾志贺氏菌1型LPS的抗体。该患者近期有前往巴基斯坦的旅行史。痢疾志贺氏菌1型的分离仍然是诊断细菌性痢疾的首选检测方法。然而，使用血清学作为提供感染痢疾志贺氏菌1型证据的手段，可能被证明是培养技术的一种有用辅助手段，但需要在该病原体为地方病的地区进行验证。
