Han Z, Stachow C
Department of Biology, Boston College, Chestnut Hill, MA 02167.
Chromosoma. 1994 Jun;103(3):162-70. doi: 10.1007/BF00368008.
The entire mitochondrial genome of Schizosaccharomyces pombe ura4-294h- was analyzed by the 2D pulsed field gel electrophoresis technique developed by Brewer and Fangman. The genome consists of multimers with an average size of 100 kb and analysis of the overlapping restriction fragments of the complete mitochondrial DNA (mtDNA) genome resulted in simple Y 2D gel patterns. Large single-stranded DNA molecules or double-stranded DNA molecules containing large or numerous single-stranded regions were found in the S. pombe mtDNA preparation. The replication of mtDNA monomers was found to occur in either direction. On the basis of these results, a replication mechanism for S. pombe mtDNA that is most consistent with a rolling circle model is suggested.
采用布鲁尔和方曼开发的二维脉冲场凝胶电泳技术，对粟酒裂殖酵母ura4 - 294h -的整个线粒体基因组进行了分析。该基因组由平均大小为100 kb的多聚体组成，对完整线粒体DNA（mtDNA）基因组重叠限制片段的分析产生了简单的Y二维凝胶图谱。在粟酒裂殖酵母mtDNA制备物中发现了大的单链DNA分子或含有大的或大量单链区域的双链DNA分子。发现mtDNA单体的复制可以向任何一个方向发生。基于这些结果，提出了一种最符合滚环模型的粟酒裂殖酵母mtDNA复制机制。
