泰国宫颈癌中人乳头瘤病毒的检测与分型

Detection and typing of human papillomavirus in cervical cancer in the Thai.

作者信息

Bhattarakosol P, Poonnaniti A, Niruthisard S

机构信息

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

出版信息

J Med Assoc Thai. 1996 Dec;79 Suppl 1:S56-64.

PMID:9071068

Abstract

One hundred formalin-fixed paraffin embedded tissues with histopathologic diagnosed invasive cervical cancer (squamous cell carcinoma) were examined for the presence of HPV-DNA by polymerase chain reaction (PCR) using L1-consensus primers. The results indicated that 82 out of 100 (82%) samples were positive for HPV-DNA. Among the positive samples, 50 samples (61%) were typed by dot hybridization technique (DH). HPV-16 was the dominant type (42.68%), followed by HPV-18 (20.73%) and HPV-33 (3.66%). There were double infection of HPV-16 and 18 in 5 (6.1%) samples. None of HPV-6 and 11 were detected in this study. This finding suggests that HPV infection is an important etiologic factor for the development of cervical cancer especially the infection with high risk types, i.e., HPV-16 and 18.

摘要

采用聚合酶链反应（PCR），使用L1共识引物，对100例经组织病理学诊断为浸润性宫颈癌（鳞状细胞癌）的福尔马林固定石蜡包埋组织进行HPV-DNA检测。结果显示，100个样本中有82个（82%）HPV-DNA呈阳性。在阳性样本中，50个样本（61%）通过点杂交技术（DH）进行分型。HPV-16是主要类型（42.68%），其次是HPV-18（20.73%）和HPV-33（3.66%）。5个样本（6.1%）存在HPV-16和18双重感染。本研究未检测到HPV-6和11。这一发现表明，HPV感染是宫颈癌发生的重要病因，尤其是高危型HPV-16和18的感染。