Hisatomi H, Miura R, Shiota K, Ogawa T, Sayama K, Tanaka S, Matsuzawa A, Kanoh Y
Department of Animal Physiology, Faculty of Agriculture, Meiji University, Kanagawa, Japan.
Jikken Dobutsu. 1994 Jul;43(3):403-8. doi: 10.1538/expanim1978.43.3_403.
Mastomys is a rodent with a intermediate size between mouse and rat, and classify as a subgenus of Praomys coucha. Our study determined the phylogenetic evolutional relationships of mastomys to mouse and rat deduced from satellite DNA sequences. Genomic DNA was extracted from each liver and digested by restriction enzymes (EcoRI and BglII). After separation and purification, we obtained these EcoRI/BglII-0.5 Kbp repeated elements and determined the nucleotide sequences. The similarities and speed of evolution were 86.06% and 0.1578 between mastomys and mouse, 85.56% and 0.1671 between mastomys and rat, and 83.91% and 0.1863 between rat and mouse, indicating that mastomys was evolutionary classified between mouse and rat, and closely related with mouse.
多乳鼠是一种体型介于小鼠和大鼠之间的啮齿动物，被归类为库氏非洲攀鼠的一个亚属。我们的研究确定了从卫星DNA序列推断出的多乳鼠与小鼠和大鼠之间的系统发育进化关系。从每个肝脏中提取基因组DNA，并用限制性内切酶（EcoRI和BglII）进行消化。经过分离和纯化后，我们获得了这些EcoRI/BglII-0.5 Kbp重复元件并确定了核苷酸序列。多乳鼠与小鼠之间的相似性和进化速度分别为86.06%和0.1578，多乳鼠与大鼠之间为85.56%和0.1671，大鼠与小鼠之间为83.91%和0.1863，这表明多乳鼠在进化上归类于小鼠和大鼠之间，且与小鼠关系密切。
