Piquette G N, Simón C, el Danasouri I, Frances A, Polan M L
Department of Gynecology and Obstetrics, Stanford University Medical Center, California.
Fertil Steril. 1994 Oct;62(4):760-70. doi: 10.1016/s0015-0282(16)57001-0.
To investigate the regulation of messenger ribonucleic acid (mRNA) levels of interleukin-1 beta (IL-1 beta), interleukin-1 (IL-1) receptor type 1, and plasminogen activator (PA) inhibitor-1 and -2 in cumulus cells, granulosa-luteal cells, and macrophage-depleted granulosa-luteal cells obtained from human preovulatory follicles.
Prospective longitudinal study.
SETTING, PATIENTS: Patients undergoing assisted reproductive technologies (ART) in the Department of Gynecology and Obstetrics, Stanford University, Stanford, California.
Cumulus cells and granulosa-luteal cells were collected by ultrasound-guided transvaginal aspiration at the time of ART.
Northern blot analysis of mRNA levels of IL-1 beta, IL-1 receptor type 1, PA inhibitor-1 and -2 in cumulus cells, granulosa-luteal cells and macrophage-depleted granulosa-luteal cells, and indirect immunocytochemical analysis of the IL-1 system and macrophages in granulosa-luteal cell preparations were performed.
Interleukin-1 beta mRNA levels in uncultured cumulus cells were less than those of uncultured granulosa-luteal cells with no differences in IL-1 receptor type 1 mRNA levels between these two cell types. Granulosa-luteal cell IL-1 receptor type 1 mRNA levels were expressed constitutively throughout 24 hours of culture with no effect by hCG, whereas IL-1 beta mRNA levels increased within 6 hours, and then remained elevated for 24 hours with no effect by hCG. Interleukin-1 beta significantly increased granulosa-luteal cell mRNA levels of IL-1 beta (over twofold), IL-1 receptor type 1 (over twofold), PA inhibitor-1 (approximately 1.4-fold), and PA inhibitor-2 (approximately 1.6-fold). In contrast, IL-1 beta had no effect on IL-1 beta and IL-1 receptor type 1 mRNA levels in macrophage-depleted granulosa-luteal cells. Granulosa-luteal cells, not macrophages, account for the majority of the immunocytochemical staining for IL-1 beta and IL-1 receptor type 1 in follicular aspirates.
These studies suggest that the IL-1 system is regulated in human granulosa-luteal cells during the periovulatory period. Furthermore, the augmentation of PA inhibitor-1 and -2 mRNA levels by IL-1 beta suggests a potential role for IL-1 beta in remodeling of the human ovary during the periovulatory period.
研究从人排卵前卵泡获取的卵丘细胞、颗粒黄体细胞及巨噬细胞缺失的颗粒黄体细胞中白细胞介素-1β（IL-1β）、白细胞介素-1（IL-1）1型受体和纤溶酶原激活物（PA）抑制剂-1及-2的信使核糖核酸（mRNA）水平的调节情况。
前瞻性纵向研究。
地点、患者：加利福尼亚州斯坦福市斯坦福大学妇产科接受辅助生殖技术（ART）的患者。
在ART时通过超声引导经阴道穿刺采集卵丘细胞和颗粒黄体细胞。
对卵丘细胞、颗粒黄体细胞及巨噬细胞缺失的颗粒黄体细胞中IL-1β、IL-1 1型受体、PA抑制剂-1及-2的mRNA水平进行Northern印迹分析，并对颗粒黄体细胞制剂中的IL-1系统和巨噬细胞进行间接免疫细胞化学分析。
未培养的卵丘细胞中IL-1β mRNA水平低于未培养的颗粒黄体细胞，这两种细胞类型的IL-1 1型受体mRNA水平无差异。颗粒黄体细胞的IL-1 1型受体mRNA水平在整个24小时培养过程中持续表达，不受人绒毛膜促性腺激素（hCG）影响，而IL-1β mRNA水平在6小时内升高，然后在24小时内保持升高，不受hCG影响。IL-1β显著增加颗粒黄体细胞中IL-1β（超过两倍）、IL-1 1型受体（超过两倍）、PA抑制剂-1（约1.4倍）和PA抑制剂-2（约1.6倍）的mRNA水平。相比之下，IL-1β对巨噬细胞缺失的颗粒黄体细胞中的IL-1β和IL-1 1型受体mRNA水平无影响。颗粒黄体细胞而非巨噬细胞是卵泡抽吸物中IL-1β和IL-1 1型受体免疫细胞化学染色的主要来源。
这些研究表明，在排卵周期前后，人颗粒黄体细胞中的IL-1系统受到调节。此外，IL-1β使PA抑制剂-1和-2的mRNA水平升高，提示IL-1β在排卵周期前后人卵巢重塑中可能发挥作用。
