Machelon V, Nome F, Durand-Gasselin I, Emilie D
Institut National de la Santé et de la Recherche Médicale (INSERM), U-355 Maturation Gamétique et Fécondation, Institut Paris-Sud sur les Cytokines, Clamart, France.
Hum Reprod. 1995 Aug;10(8):2198-203. doi: 10.1093/oxfordjournals.humrep.a136268.
Interleukin-1 beta (IL-1 beta) may be active in the ovary at ovulation and may be produced by immune and non-immune cells. This study evaluates the production of IL-1 beta by granulosa cells and macrophages from the human ovulatory follicle. The concentrations of IL-1 beta and IL-1 beta mRNA were measured in follicular aspirates taken from patients undergoing in-vitro fertilization and in cultures of cells from aspirates. Macrophages were detected by immunocytochemistry and by the reverse transcriptase polymerase chain reaction (RT-PCR). The macrophages were removed from granulosa cell preparations immediately or after the cells have been cultured for 24 h. IL-1 beta was detected by radioimmunoassay and transcripts were detected by RT-PCR and by in-situ hybridization on cytospun preparations using a [35S]IL-1 beta riboprobe. IL-1 beta and IL-1 beta mRNA were found in follicular aspirates, in granulosa luteal cell preparations containing macrophages and in highly purified granulosa cell preparations after removal of macrophages by all the methods used. Both macrophages and granulosa cells contained high concentrations of IL-1 beta transcripts. Moreover, the number of IL-1 beta reactive cells in granulosa cell preparations cultured for 24 h with 10-15% macrophages before removal was twice that of granulosa cells cultured without macrophages. Thus, both granulosa cells and macrophages are actively involved in the ovarian production of IL-1 beta at ovulation and the ability of granulosa cells to produce IL-1 beta may require ovarian macrophages.
白细胞介素-1β（IL-1β）在排卵时可能在卵巢中具有活性，且可能由免疫细胞和非免疫细胞产生。本研究评估人排卵卵泡颗粒细胞和巨噬细胞产生IL-1β的情况。在接受体外受精患者的卵泡抽吸物以及抽吸物细胞培养物中测量IL-1β和IL-1β mRNA的浓度。通过免疫细胞化学和逆转录聚合酶链反应（RT-PCR）检测巨噬细胞。巨噬细胞在颗粒细胞制备物中立即被去除，或者在细胞培养24小时后被去除。通过放射免疫测定法检测IL-1β，使用[35S]IL-1β核糖探针通过RT-PCR和细胞涂片制备物上的原位杂交检测转录本。在卵泡抽吸物、含有巨噬细胞的颗粒黄体细胞制备物以及使用所有方法去除巨噬细胞后的高度纯化颗粒细胞制备物中均发现了IL-1β和IL-1β mRNA。巨噬细胞和颗粒细胞均含有高浓度的IL-1β转录本。此外，在去除巨噬细胞之前，用10 - 15%巨噬细胞培养24小时的颗粒细胞制备物中IL-1β反应性细胞的数量是未与巨噬细胞共同培养的颗粒细胞的两倍。因此，颗粒细胞和巨噬细胞在排卵时均积极参与卵巢IL-1β的产生，且颗粒细胞产生IL-1β的能力可能需要卵巢巨噬细胞。
