Comhaire F, Schoonjans F, Vermeulen L, De Clercq N
Department of Internal Medicine, University Hospital Ghent, Belgium.
Fertil Steril. 1994 Oct;62(4):857-61. doi: 10.1016/s0015-0282(16)57016-2.
To compare the methodological accuracy of different sperm morphology criteria.
A multicenter study including 10 laboratories with high expertise in semen analysis.
Semen preparations of subfertile men with a variable degree of teratozoospermia and of fertile semen donors.
Detailed assessment of sperm morphology on 10 air-dried semen smears, of which 3 originated from the same ejaculate.
The average coefficient of variation calculated on the three smears of the same ejaculate was higher when strict criteria for morphological normality were used and borderline cells were classified as abnormal than when criteria of abnormality were applied and borderline cells were considered normal. The correspondence between individual centers mutually and of each center with the average result of all centers was better when the latter approach was taken. The performance of two computer-assisted systems was intermediate between that of the two approaches, whereas one system gave unreliable results.
Strict criteria for normality of sperm morphology, with borderline cells considered abnormal, gives results that are less reproducible and less accurate than the approach that classifies sperm as abnormal, with borderline cells considered normal.
比较不同精子形态标准的方法学准确性。
一项多中心研究，纳入了10个在精液分析方面具有高度专业知识的实验室。
患有不同程度畸形精子症的不育男性以及生育力正常的精液捐献者的精液样本。
对10张空气干燥的精液涂片进行精子形态的详细评估，其中3张涂片来自同一射精样本。
当使用严格的形态学正常标准且将临界细胞归类为异常时，同一射精样本的三张涂片计算出的平均变异系数高于应用异常标准且将临界细胞视为正常时的情况。采用后一种方法时，各中心之间以及每个中心与所有中心的平均结果之间的一致性更好。两种计算机辅助系统的性能介于两种方法之间，而其中一个系统给出的结果不可靠。
将临界细胞视为异常的严格精子形态正常标准所得到的结果，其可重复性和准确性低于将精子归类为异常且将临界细胞视为正常的方法。
