Harrington J J, Lieber M R
Department of Pathology, Stanford University School of Medicine, California 94305-5324.
Genes Dev. 1994 Jun 1;8(11):1344-55. doi: 10.1101/gad.8.11.1344.
Structure-specific nucleases catalyze critical reactions in DNA replication, recombination, and repair. Recently, a structure-specific endonuclease, FEN-1, has been purified and shown to cleave DNA flap structures. Here, we describe the cloning of the murine FEN-1 gene. The nucleotide sequence of FEN-1 is highly homologous to the Saccharomyces cerevisiae genes YKL510 and RAD2. We show that YKL510 and a truncated RAD2 protein are also structure-specific endonucleases. The substrate specificity of the truncated RAD2 protein implicates branched DNA structures as important intermediates in nucleotide excision repair. The polarity of these branched DNA structures allows us to predict the placement of DNA scissions by RAD2 and RAD1/RAD10 in this reaction.
结构特异性核酸酶催化DNA复制、重组和修复中的关键反应。最近，一种结构特异性核酸内切酶FEN-1已被纯化，并显示能切割DNA瓣状结构。在此，我们描述了小鼠FEN-1基因的克隆。FEN-1的核苷酸序列与酿酒酵母基因YKL510和RAD2高度同源。我们表明YKL510和截短的RAD2蛋白也是结构特异性核酸内切酶。截短的RAD2蛋白的底物特异性表明分支DNA结构是核苷酸切除修复中的重要中间体。这些分支DNA结构的极性使我们能够预测RAD2和RAD1/RAD10在该反应中DNA切割的位置。
