Bravo A, Hermoso J M, Salas M
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Cantoblanco, Madrid, Spain.
Gene. 1994 Oct 11;148(1):107-12. doi: 10.1016/0378-1119(94)90242-9.
Gene 3 of the Bacillus subtilis phage phi 29 encodes the terminal protein (TP), which acts as a primer in the initiation of viral DNA replication. We have developed an in vivo functional assay for the phi 29 TP based on the ability of TP-producing B. subtilis non-suppressor (su-) cells to support DNA replication of a phi 29 sus3 mutant phage. This trans-complementation assay has been used to study in vivo functional relationships between the TP of phi 29 and related phages. Our results demonstrate that phi 29 TP functionally substitutes the TP of phage PZA, whereas replication of phage Nf DNA cannot take place in vivo using the phi 29 TP as a primer.
枯草芽孢杆菌噬菌体φ29的基因3编码末端蛋白（TP），该蛋白在病毒DNA复制起始过程中充当引物。我们基于产生TP的枯草芽孢杆菌非抑制（su-）细胞支持φ29 sus3突变噬菌体DNA复制的能力，开发了一种用于φ29 TP的体内功能测定法。这种反式互补测定法已用于研究φ29与相关噬菌体的TP之间的体内功能关系。我们的结果表明，φ29 TP在功能上替代了噬菌体PZA的TP，而使用φ29 TP作为引物时，噬菌体Nf DNA的复制在体内无法发生。
