Kobayashi T
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1994 May;69(3):587-99.
Ferritin H (heavy) and L (light) subunits in red cells were determined in normal subjects, patients with myelodysplastic syndrome (MDS) and other haematological disorders by means of enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies to ferritin H and L subunits. The mean contents of ferritin H and L subunits in red cells in healthy individuals were H 8.0 + 0.8 attogram (ag)/cell (lag = 10 x 10(-18)g)(mean +/- SE), L 4.8 +/- 0.4 ag/cell respectively. The values of both subunits in normal male (H 10.5 +/- 1.3 ag/cell, L 5.9 +/- 0.7 ag/cell) were significantly higher than those of normal female (H 5.4 +/- 0.8 ag/cell, L 3.9 +/- 0.5 ag/cell). Significantly elevated H and L subunit contents in red cells were observed in patients with refractory anemia (RA)(H 138.2 +/- 72.0 ag/cell, L 57.0 +/- 20.9 ag/cell) and refractory anemia with excess of blasts (RAEB)(H 97.4 +/- 36.9 ag/cell, L 49.3 +/- 18.4 ag/cell) as compared with those of normal subjects. On the other hand, both parameters decreased in patients with iron deficiency anemia (IDA)(H 2.4 +/- 0.3 ag/cell, L 1.5 +/- 0.3 ag/cell). H/L ratio in patients with RA (2.7 +/- 0.5) was significantly higher than those of normal subjects (1.8 +/- 0.1) indicating relative increase of red cell ferritin H subunit in patient with MDS. The measurement of red cell ferritin H and L subunits by ELISA could be useful for evaluating dyserythropoiesis or ineffective hemopoiesis in MDS.
采用针对铁蛋白H和L亚基的单克隆抗体，通过酶联免疫吸附测定法（ELISA），对正常受试者、骨髓增生异常综合征（MDS）患者及其他血液系统疾病患者红细胞中的铁蛋白H（重链）和L（轻链）亚基进行了测定。健康个体红细胞中铁蛋白H和L亚基的平均含量分别为H 8.0±0.8阿托克（ag）/细胞（1 ag = 10×10⁻¹⁸克）（平均值±标准误），L 4.8±0.4 ag/细胞。正常男性的两个亚基值（H 10.5±1.3 ag/细胞，L 5.9±0.7 ag/细胞）显著高于正常女性（H 5.4±0.8 ag/细胞，L 3.9±0.5 ag/细胞）。与正常受试者相比，难治性贫血（RA）患者（H 138.2±72.0 ag/细胞，L 57.0±20.9 ag/细胞）和伴有原始细胞增多的难治性贫血（RAEB）患者（H 97.4±36.9 ag/细胞，L 49.3±18.4 ag/细胞）红细胞中的H和L亚基含量显著升高。另一方面，缺铁性贫血（IDA）患者的这两个参数均降低（H 2.4±0.3 ag/细胞，L 1.5±0.3 ag/细胞）。RA患者的H/L比值（2.7±0.5）显著高于正常受试者（1.8±0.1），表明MDS患者红细胞中铁蛋白H亚基相对增加。通过ELISA测定红细胞中铁蛋白H和L亚基，可能有助于评估MDS中的红细胞生成异常或无效造血。
