Kobayashi M, Utsunomiya T, Herndon D N, Pollard R B, Suzuki F
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555.
Immunol Lett. 1994 Apr;40(1):13-20. doi: 10.1016/0165-2478(94)90199-6.
Effect of Kanzo-bushi-to (TJS-038), one of the Japanese Kampo medicines originating from traditional Chinese herbal medicine, on the activity of a clone of burn-associated CD8+ suppressor T cells (T6S cells) was investigated. The suppressor cell activity of T6S cells in the mixed lymphocyte-tumor cell reaction (MLTR) was not changed when various concentrations of TJS-038 were added to the MLTR. The suppressor cell activity of T6S cells was greatly inhibited by CD3+ CD4+ CD28+ TCR alpha/beta+ Vicia villosa lectin-adherent cells (TJS cells) purified from spleen cells of mice treated with TJS-038 at an oral dose of 50 mg/kg. The suppressor cell activity of T6S cells was not demonstrated in the MLTR assay when anti-IL-4 monoclonal antibody was added. Also the production of IL-4 from T6S cells was not detected, when they were co-cultured with TJS cells. These results suggest that TJS-038 stimulates the generation of contrasuppressor T cells (TJS cells) that inhibit the suppressor cell activity of T6S cells and the production of IL-4 from T6S cells.
源自传统中草药的日本汉方药之一的甘草附子汤（TJS - 038）对烧伤相关CD8 +抑制性T细胞克隆（T6S细胞）活性的影响进行了研究。当向混合淋巴细胞 - 肿瘤细胞反应（MLTR）中加入不同浓度的TJS - 038时，T6S细胞在MLTR中的抑制细胞活性未发生变化。口服剂量为50 mg/kg的TJS - 038处理的小鼠脾细胞中纯化得到的CD3 + CD4 + CD28 + TCRα/β + 野豌豆凝集素粘附细胞（TJS细胞）可显著抑制T6S细胞的抑制细胞活性。在MLTR试验中，当加入抗IL - 4单克隆抗体时，未显示T6S细胞的抑制细胞活性。此外，当T6S细胞与TJS细胞共培养时，未检测到T6S细胞产生IL - 4。这些结果表明，TJS - 038刺激产生抑制T6S细胞抑制细胞活性和T6S细胞产生IL - 4的抗抑制性T细胞（TJS细胞）。
