Kobayashi M, Schmitt D A, Utsunomiya T, Pollard R B, Suzuki F
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555-0882.
Immunol Cell Biol. 1993 Jun;71 ( Pt 3):181-9. doi: 10.1038/icb.1993.20.
The inhibitory effect of glycyrrhizin (GR), an anti-inflammatory Chinese herbal drug extracted from licorice roots, on the generation of suppressor T cells in thermally injured mice (TI-mice) was investigated. The burn-associated suppressor T cell (BTs cell) activity was demonstrated in splenic mononuclear cells (SMNC) from mice 2 to 8 days after thermal injury when suppressor cell activity was assayed in a one-way mixed lymphocyte reaction. However, when TI-mice were treated with GR, SMNC harvested 6 days after thermal injury showed no suppressor cell activity. This activity of GR demonstrated a dose-response effect, with a dose of 10 mg/kg exhibiting peak levels of the activity. Since GR had no direct inactivating activities against BTs cells in vitro, the inhibitory effect of SMNC, derived from TI-mice treated with GR, on the activity of BTs cells was examined in the same mixed lymphocyte reaction system, and the results showed that the SMNC from GR-treated mice 6 days after thermal injury counteracted the activity of BTs cells. The type of cell responsible for this inhibition of BTs cell activities was a CD3+, L3T4+, Vicia villosa lectin-adherent T cell with the same phenotypic properties previously exhibited by contrasuppressor cells. These results suggest that GR may regulate the generation of BTs cells through the induction of contrasuppressor cells. Since there are many reports describing septic infections due to the appearance of BTs cells in postburn patients, it may be possible to apply GR or blood preparations containing contrasuppressor cell populations induced by GR in healthy volunteers into immunosuppressed burn patients to avoid infections.
研究了从甘草根中提取的抗炎中草药甘草甜素（GR）对热损伤小鼠（TI-小鼠）中抑制性T细胞生成的抑制作用。在热损伤后2至8天的小鼠脾单核细胞（SMNC）中，当在单向混合淋巴细胞反应中测定抑制细胞活性时，证明了烧伤相关抑制性T细胞（BTs细胞）活性。然而，当TI-小鼠用GR治疗时，热损伤后6天收获的SMNC未显示出抑制细胞活性。GR的这种活性表现出剂量反应效应，10mg/kg的剂量表现出该活性的峰值水平。由于GR在体外对BTs细胞没有直接的灭活活性，因此在相同的混合淋巴细胞反应系统中检测了用GR处理的TI-小鼠来源的SMNC对BTs细胞活性的抑制作用，结果表明，热损伤后6天来自GR处理小鼠的SMNC抵消了BTs细胞的活性。负责抑制BTs细胞活性的细胞类型是CD3 +、L3T4 +、野豌豆凝集素粘附T细胞，其具有先前反抑制细胞所表现出的相同表型特性。这些结果表明，GR可能通过诱导反抑制细胞来调节BTs细胞的生成。由于有许多报道描述了烧伤后患者中由于BTs细胞的出现而导致的败血症感染，因此有可能将GR或含有由GR诱导的反抑制细胞群体的血液制剂应用于健康志愿者中的免疫抑制烧伤患者以避免感染。
