Holcman B, Heath D D, Shaw R J
Dpto. Zoología Invertebrados, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Int J Parasitol. 1994 Aug;24(5):623-35. doi: 10.1016/0020-7519(94)90114-7.
The ultrastructure of the oncospheres and developing metacestodes in vitro of Echinococcus granulosus was studied with emphasis on the origin of the laminated layer. The activated oncospheres measured 28 microns (S.D. = 1.83), and by day 5 of in vitro culture the metacestodes attained diameters up to 52 microns (S.D. = 2.66). The oncospheral plasma membrane of the tegument appeared to be formed by a microtubular cytoskeleton arranged in a predetermined pattern. By day 2 there were three main types of vesicles in the perikaryon: Vd, Vg1 and Vg2. Vesicles appeared to be synthesized in the perikaryon and continuously transported to the periphery, where the Vd vesicles could contribute to the ground substance of the syncytial tegument. We suggest that the Vg1 and Vg2 vesicles contribute to the laminated layer where, by day 5, they were seen in increased density and forming aggregations on the outer border.
对细粒棘球绦虫六钩蚴及体外发育的中期绦虫的超微结构进行了研究，重点关注分层层的起源。活化的六钩蚴大小为28微米（标准差=1.83），体外培养第5天时，中期绦虫的直径可达52微米（标准差=2.66）。皮层的六钩蚴质膜似乎由按预定模式排列的微管细胞骨架形成。到第2天时，核周有三种主要类型的囊泡：Vd、Vg1和Vg2。囊泡似乎在核周合成，并不断运输到外周，在那里Vd囊泡可形成合胞体皮层的基质。我们认为Vg1和Vg2囊泡有助于形成分层层，到第5天时，它们在外边界的密度增加并形成聚集。
