Naz R K, Kaplan P
Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York 10461.
J Androl. 1994 May-Jun;15(3):220-7.
The presence of various cytokines, namely the tumor necrosis factor (TNF-alpha), interferon (IFN-gamma), and interleukins (IL-1 beta and IL-6), was investigated in seminal plasma of fertile, infertile, and immunoinfertile men using specific immunoradiometric assays. TNF-alpha and IL-1 beta were not detected. IFN-gamma was detected, but the differences between the levels of fertile and infertile/immunoinfertile were not significant (P > 0.05). IL-6 was detected in seminal plasma with significantly higher levels in infertile/immunoinfertile men compared to those of fertile men. IL-6 was also present in sera, and interestingly, the levels in sera were lower than those in seminal plasma. IL-6 levels in seminal plasma correlated significantly with some sperm parameters and penetration rates in the human sperm penetration assay (SPA). These findings suggest that IL-6 is associated with infertility and may be of importance in specific diagnosis and treatment of male infertility.
使用特异性免疫放射分析方法，对生育能力正常、不育和免疫性不育男性的精浆中多种细胞因子，即肿瘤坏死因子（TNF-α）、干扰素（IFN-γ）和白细胞介素（IL-1β和IL-6）的存在情况进行了研究。未检测到TNF-α和IL-1β。检测到了IFN-γ，但生育能力正常者与不育/免疫性不育者之间的水平差异不显著（P>0.05）。在精浆中检测到了IL-6，不育/免疫性不育男性的IL-6水平显著高于生育能力正常的男性。血清中也存在IL-6，有趣的是，血清中的水平低于精浆中的水平。精浆中的IL-6水平与人类精子穿透试验（SPA）中的一些精子参数和穿透率显著相关。这些发现表明，IL-6与不育有关，可能在男性不育的特异性诊断和治疗中具有重要意义。
